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Biomechanical and proteomic analysis of INF-beta-treated astrocytes

机译：INF-β处理的星形胶质细胞的生物力学和蛋白质组学分析

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Astrocytes have a key role in the pathogenesis of several diseases including multiple sclerosis and were proposed as the designed target for immunotherapy. In this study we used atomic force microscopy (AFM) and proteomics methods to analyse and correlate the modifications induced in the viscoleastic properties of astrocytes to the changes induced in protein expression after interferon-beta (IFN-beta) treatment. Our results indicated that IFN-beta treatment resulted in a significant decrease in the Young's modulus, a measure of cell elasticity, in comparison with control cells. The molecular mechanisms that trigger these changes were investigated by 2DE (two-dimensional electrophoresis) and confocal analyses and confirmed by western blotting. Altered proteins were found to be involved in cytoskeleton organization and other important physiological processes.
机译：星形胶质细胞在包括多发性硬化症在内的多种疾病的发病机理中具有关键作用，并被提议作为免疫疗法的设计目标。在这项研究中，我们使用原子力显微镜（AFM）和蛋白质组学方法来分析和关联在星形胶质细胞的粘弹性特性中诱导的修饰与干扰素-β（IFN-β）处理后诱导的蛋白质表达变化。我们的结果表明，与对照细胞相比，IFN-β处理导致杨氏模量（一种衡量细胞弹性的指标）显着降低。触发这些变化的分子机制已通过2DE（二维电泳）和共聚焦分析进行了研究，并通过Western印迹得到了证实。发现改变的蛋白质与细胞骨架组织和其他重要的生理过程有关。

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