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ViriChip: a solid phase assay for detection and identification of viruses by atomic force microscopy

机译：ViriChip：固相分析，通过原子力显微镜检测和鉴定病毒

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Bionanotechnology can be viewed as the integration of tools and concepts in nanotechnology with the attributes of biomolecules. We report here on an atomic force microscopy-immunosensor assay (AFMIA) that couples AFM with solid phase affinity capture of biological entities for the rapid detection and identification of group B coxsackievirus particles. Virus identification is based on type-specific immunocapture and the morphological properties of the captured viruses as obtained by the AFM. Representatives of the six group B coxsackieviruses have been specifically captured from I mul volumes of clarified cell lysates, body fluids and environmental samples. Concentration and kinetic profiles for capture indicate that detection is possible at 10~3 TCID~(50) mul~(-1) and the dynamic range of the assay spans three logs. The results demonstrate that the melding of a nanotechnological tool (AFM) with biotechnology (solid phase immunocapture of virus particles) can create a clinically relevant platform, useful for the detection and identification of enterovirus particles in a variety of samples.
机译：生物纳米技术可以看作是纳米技术中具有生物分子属性的工具和概念的集成。我们在这里报告原子力显微镜免疫传感器测定法（AFMIA），该方法将AFM与生物实体的固相亲和力捕获相结合，用于快速检测和鉴定B组柯萨奇病毒颗粒。病毒识别基于类型特异性免疫捕获和通过AFM获得的捕获病毒的形态学特征。六种B组柯萨奇病毒的代表已从大量的澄清细胞裂解液，体液和环境样品中捕获。捕获的浓度和动力学曲线表明，在10〜3 TCID〜（50）mul〜（-1）时可以进行检测，并且测定的动态范围跨越了三个对数。结果表明，纳米技术工具（AFM）与生物技术（病毒颗粒的固相免疫捕获）的融合可以创建临床相关平台，可用于检测和鉴定各种样品中的肠道病毒颗粒。

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