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Preparation of quantum dot-coated magnetic polystyrene nanospheres for cancer cell labelling and separation

机译:量子点包覆磁性聚苯乙烯纳米球的制备

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CdTe-coated magnetic polystyrene nanospheres (MPN) were prepared via a stepwise electrostatic self-assembly approach, and the conjugation of epidermal growth factor (EGF) to the MPN/CdTe core - shell nanocomposites was prepared by using 1-ethyl-3(3-dimethylamino propyl)- carbodiimide (EDC) as a cross-linking reagent. The MPN/CdTe and their bioconjugates yielded not only emitted bright fluorescence, but also exhibited superparamagnetism. The human breast cancer MDA-MB-435S cells could be labelled and rapidly separated by the MPN/CdTe - EGF bioconjugates. These magnetofluorescent nanospheres, consisting of magnetic spheres and quantum dots (QDs), may be of special interest for many biomedical applications.
机译:通过逐步静电自组装方法制备了包覆CdTe的磁性聚苯乙烯纳米球(MPN),并使用1-乙基-3(3)制备了表皮生长因子(EGF)与MPN / CdTe核-壳纳米复合物的结合。 -二甲氨基丙基)-碳二亚胺(EDC)作为交联剂。 MPN / CdTe及其生物共轭物不仅发出明亮的荧光,而且表现出超顺磁性。人乳腺癌MDA-MB-435S细胞可以被MPN / CdTe-EGF生物缀合物标记并快速分离。这些由磁球和量子点(QD)组成的磁荧光纳米球可能对许多生物医学应用特别感兴趣。

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