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首页> 外文期刊>Nanotechnology >Green synthesis of highly stabilized nanocrystalline silver particles by a non-pathogenic and agriculturally important fungus T-asperellum
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Green synthesis of highly stabilized nanocrystalline silver particles by a non-pathogenic and agriculturally important fungus T-asperellum

机译：非病原性和农业上重要的真菌T-绿色真菌绿色合成高度稳定的纳米晶银颗粒

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摘要

A controlled and up-scalable biosynthetic route to nanocrystalline silver particles with well-defined morphology using cell-free aqueous filtrate of a non-pathogenic and commercially viable biocontrol agent Trichoderma asperellum is being reported for the first time. A transparent solution of the cell-free filtrate of Trichoderma asperellum containing 1 mM AgNO3 turns progressively dark brown within 5 d of incubation at 25 degrees C. The kinetics of the reaction was studied using UV-vis spectroscopy. An intense surface plasmon resonance band at similar to 410 nm in the UV-vis spectrum clearly reveals the formation of silver nanoparticles. The size of the silver particles using TEM and XRD studies is found to be in the range 13-18 nm. These nanoparticles are found to be highly stable and even after prolonged storage for over 6 months they do not show significant aggregation. A plausible mechanism behind the formation of silver nanoparticles and their stabilization via capping has been investigated using FTIR and surface-enhanced resonance Raman spectroscopy.
机译：首次报道了使用无病原性和商业上可行的生物控制剂曲霉木霉的无细胞含水滤液，以可控制的，可扩展的生物合成路线来形成形态明确的纳米晶银颗粒。含1 mM AgNO3的无木霉菌无细胞滤液的透明溶液在25°C下孵育5 d内逐渐变成深棕色。使用UV-vis光谱学研究了反应动力学。紫外可见光谱中类似于410 nm的强烈表面等离子体共振带清楚地揭示了银纳米颗粒的形成。使用TEM和XRD研究发现银颗粒的尺寸在13-18nm范围内。发现这些纳米颗粒是高度稳定的，甚至在长时间存储超过6个月后，它们也没有显示出明显的聚集。已经使用FTIR和表面增强共振拉曼光谱研究了银纳米颗粒形成及其通过封端稳定的合理机制。

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