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Fluctuation correlation spectroscopy and photon histogram analysis of light scattered by gold nanospheres

机译：金纳米球散射光的涨落相关光谱和光子直方图分析

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Fluorescence correlation spectroscopy (FCS) is a valuable tool in biological research. In recent years there has been growing interest in using light scattered from metallic colloids in place of organic fluorophores. Metallic colloids display optical cross sections for scattering that are orders of magnitude brighter than fluorophores. We used the FCS method to study the scattering properties of varying sizes of gold colloids 38-100 nm in diameter. The optical cross sections of the gold colloids increase rapidly with size, as can be seen by both the G(0) value of the autocorrelation function and the scattering intensity distributions. In mixtures of different size gold colloids the autocorrelation function is dominated by the larger (brighter) colloids, even when present at a small fractional population. We show that it is possible to detect one 100 nm gold colloid in the presence of 10~3-10~4 smaller 39 nm diameter colloids. Because the scattering cross sections of colloids will increase with aggregation, we believe that FCS can be used to detect a small number of associated bio-labeled colloids in the presence of a much larger population of non-associated colloids.
机译：荧光相关光谱法（FCS）是生物学研究中的宝贵工具。近年来，人们越来越关注使用从金属胶体散射的光代替有机荧光团。金属胶体显示的光学散射截面比荧光团亮几个数量级。我们使用FCS方法研究了直径38-100 nm的各种大小的金胶体的散射特性。从自相关函数的G（0）值和散射强度分布可以看出，金胶体的光学截面随尺寸的增加而迅速增加。在不同大小的金胶体混合物中，自相关函数由较大的（较亮的）胶体主导，即使存在的比例很小。我们表明，在存在10〜3-10〜4个较小的39 nm直径的胶体的情况下，可以检测到一种100 nm的金胶体。由于胶体的散射截面会随着聚集而增加，因此我们认为，在存在大量非缔合胶体的情况下，FCS可用于检测少量关联的生物标记胶体。

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