Selectivity improvement in protein nanopatterning with a hydroxy-terminated self-assembled monolayer template

Miyake T; Tanii T; Kato K; Zako T; Funatsu T; Ohdomari I

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Selectivity improvement in protein nanopatterning with a hydroxy-terminated self-assembled monolayer template

机译：羟基端接的自组装单层模板提高蛋白质纳米图案的选择性

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Protein nanopatterning techniques to fulfil the requirement for reducing nonspecific adsorption are demonstrated. A target protein is selectively immobilized on a hydroxy-terminated self-assembled monolayer template, the pattern of which is modified with streptavidin used as the intermediating molecule. 3-aminopropyltrimethoxysilane (APTES) used as the intermediating molecule induces nonspecific adsorption of proteins due to nonspecific adsorption of APTES itself. The data indicate that reducing nonspecific adsorption of both the target protein and the intermediating molecule is important for selectivity improvement in protein patterning. As a result of the refinement, a protein nanopattern of 250 nm in diameter has been fabricated.

机译：蛋白质纳米图案技术可以满足减少非特异性吸附的要求。将靶蛋白选择性地固定在羟基末端的自组装单层模板上，该模板的图案被链霉亲和素用作中间分子修饰。由于APTES本身的非特异性吸附，用作中间分子的3-氨基丙基三甲氧基硅烷（APTES）诱导了蛋白质的非特异性吸附。数据表明，减少靶蛋白和中间分子的非特异性吸附对于提高蛋白构图的选择性很重要。作为改进的结果，已经制造了直径为250nm的蛋白质纳米图案。

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《Nanotechnology》 |2007年第30期|共6页
作者
Miyake T; Tanii T; Kato K; Zako T; Funatsu T; Ohdomari I;
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正文语种 eng
中图分类一般工业技术;
关键词
ELECTRON-BEAM LITHOGRAPHY; IMMOBILIZATION; BIOMOLECULES; NANOARRAYS; ADHESION; RESIST;

机译：电子束光刻;固定化;生物分子;纳米阵列;附着力;抗性;

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1. Selectivity improvement in protein nanopatterning with a hydroxy-terminated self-assembled monolayer template [J] . Miyake T, Tanii T, Kato K, Nanotechnology . 2007,第30期

机译：羟基端接的自组装单层模板提高蛋白质纳米图案的选择性
2. Nanopatterning of hydroxy-terminated self-assembled monolayer taking advantage of terminal group modification [J] . Miyake T, Tanii T, Kato K, Chemical Physics Letters . 2006,第4a6期

机译：利用端基修饰技术对羟基封端的自组装单分子膜进行纳米构图
3. Fabrication of chemical templates via selective laser-induced desorption of hexadecanethiol self-assembled monolayers [J] . Anja Schroeter, Mareike Mathieu, Steffen Franzka, Applied Surface Science . 2013,第auga1期

机译：通过选择性激光诱导的十六烷硫醇自组装单分子层的解吸制备化学模板
4. Macrophage Adhesion to CH3-, OH- and EG4-terminated Self-Assembled Monolayers: influence of serum proteins and adsorbed fibronectin [C] . J. Maciel, M. Oliveira, M. C. L. Martins, 23rd European conference on biomaterials 2010 . 2010

机译：巨噬细胞对CH3-，OH-和EG4终止的自组装单层的粘附：血清蛋白和吸附的纤连蛋白的影响
5. Nanopatterning of recombinant proteins and viruses using block copolymer templates. [D] . Cresce, Arthur von Wald. 2007

机译：使用嵌段共聚物模板对重组蛋白和病毒进行纳米图案化。
6. Near-field photochemical and radiation-induced chemical fabrication of nanopatterns of a self-assembled silane monolayer [O] . Ulrich Christian Fischer, Carsten Hentschel, Florian Fontein, 2014

机译：自组装硅烷单层纳米图案的近场光化学和辐射诱导化学制备
7. Site-Selective Deposition and Micropatterning of Zirconia Thin Films on Templates of Self-Assembled Monolayers [O] . Yanfeng GAO, Yoshitake MASUDA, Tetsu YONEZAWA, 2002

机译：自组装单层模板上氧化锆薄膜的站点选择性沉积和微图案
8. Polymeric Self-Assembled Monolayers. 4. Synthesis, Characterization, andStability of omega-Functionalized, Self-Assembled Diacetylenic and Polydiacetylenic Monolayers [R] . Kim, T., Chan, K. C., Crooks, R. M., 1996

机译：聚合物自组装单层膜。 4.ω-功能化的，自组装的二乙炔和聚二乙炔单分子层的合成，表征和稳定性

Selectivity improvement in protein nanopatterning with a hydroxy-terminated self-assembled monolayer template

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