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首页> 外文期刊>Nanotechnology >Poly(N-isopropylacrylamide)-coated thermo-responsive nanoparticles for controlled delivery of sulfonated Zn-phthalocyanine in Chinese hamster ovary cells in vitro and zebra fish in vivo
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Poly(N-isopropylacrylamide)-coated thermo-responsive nanoparticles for controlled delivery of sulfonated Zn-phthalocyanine in Chinese hamster ovary cells in vitro and zebra fish in vivo

机译：聚（N-异丙基丙烯酰胺）涂层的热响应纳米颗粒可控制磺化锌酞菁在体外向中国仓鼠卵巢细胞和体内斑马鱼的递送

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摘要

Poly( N- isopropylacrylamide) ( PNIPAM)- coated Fe3O4@ SiO2@ CdTe multifunctional nanoparticles with photoluminescent ( PL), thermosensitive and magnetic properties, were investigated as carriers to deliver water- soluble, fluorescent sulfonated Zn- phthalocyanine ( ZnPcS), a photosensitizing drug for photodynamic therapy of cancer, in Chinese hamster ovary ( CHO) cells in vitro and zebra fish in vivo. PNIPAM is a well- known thermo- responsive polymer with a volume phase transition temperature. This property allows it to be swollen in water at temperatures lower than 32 - 34 degrees C to take up ZnPcS and shrunken to expel the drug at higher temperatures. Since the PL band of CdTe quantum dots ( QDs) as indicators for the nanoparticles is at 585 nm and the emission band of ZnPcS is at 680 nm, it is possible to study the temperature- dependent release of ZnPcS from the nanoparticles by fluorescence measurements. ZnPcS was embedded in the PNIPAM of the nanoparticles at 25 degrees C in phosphate buffered saline ( PBS) solution and released at 37 degrees C, measured with a spectrophotometer. When CHO cells had been incubated with the ZnPcS- loaded nanoparticles at 27 degrees C, a similar intracellular localization pattern of CdTe QDs and ZnPcS was seen by multichannel measurements in confocal laser scanning microscopy ( CLSM), but a diffuse pattern of only ZnPcS fluorescence was detected in the cytoplasm of the cells at 37 degrees C, indicating a release of ZnPcS from the nanoparticles. Similar results were also found in the intestinal tract of zebra fish in vivo after intake of the nanoparticles. Since the nanoparticles contain magnetic ( Fe3O4) material, the nanoparticles could also be manipulated to change their location in the intestinal tract of the zebra fish with an external magnetic field gradient of 300 G mm(-1). The results presented suggest that such multifunctional nanoparticles may have combined potential for temperature- dependent drug delivery, QD photodetection and magnetic manipulation in diagnosis and therapy of diseases.
机译：研究了聚（N-异丙基丙烯酰胺）（PNIPAM）包覆的具有光致发光（PL），热敏和磁性性质的Fe3O4 @ SiO2 @ CdTe多功能纳米粒子作为载体提供水溶性，荧光磺化的锌酞菁（ZnPcS）的载体。在体外中国仓鼠卵巢（CHO）细胞和体内斑马鱼中进行光动力疗法治疗癌症的药物。 PNIPAM是一种具有体积相变温度的众所周知的热响应聚合物。该特性使它在低于32-34摄氏度的温度下在水中溶胀，吸收ZnPcS，并在更高的温度下收缩而排出药物。由于作为纳米颗粒指示剂的CdTe量子点（QDs）的PL带在585 nm处，而ZnPcS的发射带在680 nm处，因此有可能通过荧光测量研究ZnPcS从纳米颗粒的温度依赖性释放。 ZnPcS在25摄氏度的磷酸盐缓冲盐溶液（PBS）溶液中嵌入纳米颗粒的PNIPAM中，并在37摄氏度下用分光光度计测量释放。当CHO细胞与载有ZnPcS的纳米颗粒在27摄氏度下孵育时，通过共聚焦激光扫描显微镜（CLSM）中的多通道测量可以观察到CdTe QD和ZnPcS的细胞内定位模式相似，但只有ZnPcS荧光的扩散模式为在37℃下在细胞质中检测到ZnPcS，表明从纳米颗粒释放了ZnPcS。摄入纳米颗粒后，在体内斑马鱼的肠道中也发现了类似的结果。由于纳米粒子包含磁性（Fe3O4）材料，因此还可以操纵纳米粒子以300 G mm（-1）的外部磁场梯度改变它们在斑马鱼肠道中的位置。提出的结果表明，这种多功能纳米颗粒在疾病的诊断和治疗中可能具有依赖温度的药物递送，QD光检测和磁操作的综合潜力。

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