Observation of microtubules with scanning force microscopy in liquid

P J de Pablo; I A T Schaap; C F Schmidt

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Observation of microtubules with scanning force microscopy in liquid

机译：扫描力显微镜在液体中观察微管

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We present the application of scanning force microscopy using the jumping mode to investigate microtubules adsorbed to glass in air and in liquid. To fix the microtubules the glass surfaces were silanized with aminopropyl-triethoxy-silane. The observed structures ranged from disrupted microtubules in air to intact microtubules in liquid. Intact microtubules show heights between 20 and 24 nm confirming the diameter found in electron microscopy studies. The force applied by the tip was critical for the microtubule height, indicating deformation by the tip. Internal structure, corresponding to protofilaments, was found.

机译：我们提出使用跳跃模式的扫描力显微镜的应用，以研究微管在空气和液体中吸附到玻璃上的情况。为了固定微管，将玻璃表面用氨基丙基-三乙氧基-硅烷硅烷化。观察到的结构从空气中的微管破裂到液体中的完整微管不等。完整的微管显示出20至24 nm的高度，从而证实了在电子显微镜研究中发现的直径。尖端施加的力对于微管高度至关重要，表明尖端变形。发现了与原丝相对应的内部结构。

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《Nanotechnology》 |2003年第2期|共4页
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P J de Pablo; I A T Schaap; C F Schmidt;
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1. Observation of microtubules with scanning force microscopy in liquid [J] . P J de Pablo, I A T Schaap, C F Schmidt Nanotechnology . 2003,第2期

机译：扫描力显微镜在液体中观察微管
2. SCANNING FORCE MICROSCOPY OF MICROTUBULES AND POLYMORPHIC TUBULIN ASSEMBLIES IN AIR AND IN LIQUID [J] . Vater W., Schaper A., Bohm KJ., Journal of Cell Science . 1995,第Pta3期

机译：空气和液体中微管和多态微管蛋白组件的扫描力显微镜
3. Cross-sectional observation in nanoscale for Si power MOSFET by atomic force microscopy/Kelvin probe force microscopy/scanning capacitance force microscopy [J] . Doi Atsushi, Nakajima Mizuki, Masuda Sho, Japanese journal of applied physics . 2019,第期

机译：用原子力显微镜/开尔文探头力显微镜/扫描电容力显微镜通过原子力显微镜MOSFET在纳米级横截面观察
4. Application of scanning force microscopy and near-field optical microscopy to liquid crystalline systems: observing free surfaces smectic structural forces and molecular orientation [C] . Author(s): Ricky L. Williamson Univ. of Bristol Bristol United Kingdom, M.Rivera Univ. of Bristol Bristol United Kingdom, M.J. Miles Univ. of Bristol Bristol United Kingdom, Scanning Probe Microscopies III . 1995

机译：扫描力显微镜和近场光学显微镜在液晶系统中的应用：观察自由表面近晶结构力和分子取向
5. Scanning electrochemical microscopy of liquid/liquid and liquid/membrane interfaces. [D] . Liu, Biao. 2002

机译：液体/液体和液体/膜界面的扫描电化学显微镜。
6. Simple observation of Streptococcus mutans biofilm by scanning electron microscopy using ionic liquids [O] . Yoko Asahi, Jiro Miura, Tetsuya Tsuda, 2015

机译：使用离子液体的扫描电子显微镜简单观察变异链球菌的生物膜
7. Liquid‐Cell Electron Microscopy: Observation of Undamped 3D Brownian Motion of Nanoparticles Using Liquid‐Cell Scanning Transmission Electron Microscopy (Part. Part. Syst. Charact. 6/2020) [O] . Tom A. J. Welling, Sina Sadighikia, Kanako Watanabe, 2020

机译：液体电子显微镜：使用液体扫描透射电子显微镜观察纳米粒子的纳米颗粒的稳定3D褐色运动（部分。第7部分。SYST。挑战。6/2020）
8. Scanning Tunneling Microscopy and Atomic Force Microscopy of the Liquid-Solid Interface [R] . Schneir, J., Marti, O., Remmers, G., 1988

机译：扫描隧道显微镜和液 - 固界面的原子力显微镜

Observation of microtubules with scanning force microscopy in liquid

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