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The effects of gold nanoparticles with different sizes on polymerase chain reaction efficiency

机译:不同大小金纳米颗粒对聚合酶链反应效率的影响

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We report the effect of 5, 10 and 20 nm gold nanoparticles (AuNP_s) on polymerase chain reaction (PCR) efficiency and a proposed mechanism for AuNPs affecting the PCR. It is observed that AuNPs can cause PCR inhibition, the degree of which is affected by the concentration of the AuNPs. AuNPs of larger sizes can cause complete PCR inhibition at a lower particle concentration than those of smaller sizes. Evidence from different experiments suggests that a probable mechanism is through polymerase–AuNP binding. The collected data show that the product yield is modulated by the total surface area of the AuNPs regardless of the size, which further supports the hypothesis.
机译:我们报告了5,10和20 nm的金纳米颗粒(AuNP_s)对聚合酶链反应(PCR)效率的影响以及AuNPs影响PCR的拟议机制。观察到AuNPs可引起PCR抑制,其程度受AuNPs浓度影响。与较小尺寸的AuNPs相比,较大尺寸的AuNPs在较低的颗粒浓度下可引起完全的PCR抑制。来自不同实验的证据表明,可能的机制是通过聚合酶与AuNP的结合。收集到的数据表明,无论大小如何,AuNPs的总表面积都会调节产物的收率,这进一步支持了这一假设。

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