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A dual-colored bio-marker made of doped ZnO nanocrystals

机译：掺杂的ZnO纳米晶体制成的双色生物标记

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Bio-compatible ZnO nanocrystals doped with Co, Cu and Ni cations, surface capped with two types of aminosilanes and titania are synthesized by a soft chemical process. Due to the small particle size (2-5 nm), surface functional groups and the high photoluminescence emissions at the UV and blue-violet wavelength ranges, bio-imaging on human osteosarcoma (Mg-63) cells and histiocytic lymphoma U-937 monocyte cells showed blue emission at the nucleus and bright turquoise emission at the cytoplasm simultaneously. This is the first report on dual-color bio-images labeled by one semiconductor nanocrystal colloidal solution. Bright green emission was detected on mung bean seedlings labeled by all the synthesized ZnO nanocrystals. Cytotoxicity tests showed that the aminosilanes capped nanoparticles are non-toxic. Quantum yields of the nanocrystals varied from 79% to 95%. The results showed the potential of the pure ZnO and Co-doped ZnO nanocrystals for live imaging of both human cells and plant systems.
机译：通过软化学工艺合成了掺有Co，Cu和Ni阳离子的生物相容性ZnO纳米晶体，表面包覆了两种类型的氨基硅烷和二氧化钛。由于粒径小（2-5 nm），表面官能团以及在紫外线和蓝紫色波长范围内的高光致发光发射，对人骨肉瘤（Mg-63）细胞和组织细胞性淋巴瘤U-937单核细胞的生物成像细胞在细胞核同时显示蓝色发射，在细胞质同时显示明亮的绿松石发射。这是关于用一种半导体纳米晶体胶体溶液标记的双色生物图像的第一份报告。在所有合成的ZnO纳米晶体标记的绿豆幼苗上检测到亮绿色的发射光。细胞毒性测试表明，氨基硅烷封端的纳米颗粒是无毒的。纳米晶体的量子产率在79％至95％之间变化。结果表明纯ZnO和Co掺杂的ZnO纳米晶体具有用于人体细胞和植物系统实时成像的潜力。

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