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Functional single-walled carbon nanotubes based on an integrin a_v,__3 monoclonal antibody for highly efficient cancer cell targeting

机译：基于整联蛋白a_v，__ 3单克隆抗体的功能性单壁碳纳米管，可高效靶向癌细胞

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The application of single-walled carbon nanotubes (SWNTs) in the field of biomedicine is becoming an entirely new and exciting topic. In this study, a novel functional SWNT based on an integrin a_vβ_3 monoclonal antibody was developed and was used for cancer cell targeting in vitro. SWNTs were first modified by phospholipid-bearing polyethylene glycol (PL_PEG). The PL_PEG functionalized SWNTs were then conjugated with protein A. A SWNT_integrin a_v__3 monoclonal antibody system (SWNT_PEG_mAb) was thus constructed by conjugating protein A with the fluorescein labeled integrin a_v__3 monoclonal antibody. In vitro study revealed that SWNT_PEG_mAb presented a high targeting efficiency on integrin α_v__3-positive U87MG cells with low cellular toxicity, while for integrin α_v__3-negative MCF-7 cells, the system had a low targeting efficiency, indicating that the high targeting to U87MG cells was due to the specific integrin targeting of the monoclonal antibody. In conclusion, SWNT_PEG_mAb developed in this research is a potential candidate for cancer imaging and drug delivery in cancer targeting therapy.
机译：单壁碳纳米管（SWNTs）在生物医学领域的应用正在成为一个全新的令人兴奋的话题。在这项研究中，开发了一种基于整合素α_vβ_3单克隆抗体的新型功能性SWNT，并将其用于体外靶向癌细胞。首先通过含磷脂的聚乙二醇（PL_PEG）修饰SWNT。然后将PL_PEG功能化的SWNT与蛋白A偶联。通过将蛋白A与荧光素标记的整联蛋白a_v__3单克隆抗体缀合，从而构建SWNT_整联蛋白a_v__3单克隆抗体系统（SWNT_PEG_mAb）。体外研究表明，SWNT_PEG_mAb对具有低细胞毒性的整联蛋白α_v__3阳性的U87MG细胞具有较高的靶向效率，而对于整联蛋白α_v__3阴性的MCF-7细胞，该系统具有较低的靶向效率，表明对U87MG细胞的靶向性较高这是由于单克隆抗体的特异性整联蛋白靶向。总之，在这项研究中开发的SWNT_PEG_mAb在癌症靶向治疗中是癌症成像和药物输送的潜在候选者。

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