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首页> 外文期刊>Nanotechnology >A simple gold nanoparticle-mediated immobilization method to fabricate highly homogeneous DNA microarrays having higher capacities than those prepared by using conventional techniques
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A simple gold nanoparticle-mediated immobilization method to fabricate highly homogeneous DNA microarrays having higher capacities than those prepared by using conventional techniques

机译：一种简单的金纳米粒子介导的固定化方法，以制造比使用常规技术制备的具有更高容量的高度均质的DNA微阵列

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摘要

A simple, highly efficient immobilization method to fabricate DNA microarrays, that utilizes gold nanoparticles as the mediator, has been developed. The fabrication method begins with electrostatic attachment of amine-modified DNA to gold nanoparticles. The resulting gold-DNA complexes are immobilized on conventional amine or aldehyde functionalized glass slides. By employing gold nanoparticles as the immobilization mediator, implementation of this procedure yields highly homogeneous microarrays that have higher binding capacities than those produced by conventional methods. This outcome is due to the increased three-dimensional immobilization surface provided by the gold nanoparticles as well as the intrinsic effects of gold on emission properties. This novel immobilization strategy gives microarrays that produce more intense hybridization signals for the complementary DNA. Furthermore, the silver enhancement technique, made possible only in the case of immobilized gold nanoparticles on the microarrays, enables simple monitoring of the integrity of the immobilized DNA probe.
机译：已经开发出一种简单，高效的固定方法来制造DNA微阵列，该方法利用金纳米颗粒作为介体。制备方法开始于将胺修饰的DNA静电附着到金纳米颗粒上。将所得的金-DNA复合物固定在常规的胺或醛官能化的载玻片上。通过使用金纳米颗粒作为固定介体，该程序的实施产生了高度均一的微阵列，其具有比常规方法产生的结合能力更高的结合能力。该结果是由于金纳米颗粒提供的三维固定化表面增加以及金对发射特性的内在影响。这种新颖的固定策略使微阵列能够为互补DNA产生更强烈的杂交信号。此外，仅在将金纳米颗粒固定在微阵列上的情况下才有可能使用银增强技术，从而可以简单地监控固定的DNA探针的完整性。

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