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首页> 外文期刊>Nanotechnology >Intercalating dye as an acceptor in quantum-dot-mediated FRET
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Intercalating dye as an acceptor in quantum-dot-mediated FRET

机译：嵌入染料作为量子点介导的FRET中的受体

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摘要

Fluorescence resonance energy transfer (FRET) is a popular tool to study intermolecular distances and characterize structural or conformational changes of biological macromolecules. We investigate a novel inorganic/organic FRET pair with quantum dots (QDs) as donors and DNA intercalating dyes, BOBO-3, as acceptors by using DNA as a linker. Typically, FRET efficiency increases with the number of stained DNA linked to a QD. However, with the use of intercalating dyes, we demonstrate that FRET efficiency at a fixed DNA: QD ratio can be further enhanced by increasing the number of dyes stained to a DNA strand through the use of an increased staining dye/bp ratio. We exploit this flexibility in the staining ratio to maintain a high FRET efficiency of > 0.90 despite a sixfold decrease in DNA concentration. Having characterized this new QD-mediated FRET system, we test this system in a cellular environment using nanocomplexes generated by encapsulating DNA with commercial non-viral gene carriers. Using this novel FRET pair, we are able to monitor the configuration changes and fate of the DNA nanocomplexes during intracellular delivery, thereby providing an insight into the mechanistic study of gene delivery.
机译：荧光共振能量转移（FRET）是研究分子间距离并表征生物大分子结构或构象变化的流行工具。我们研究了一种新型的无机/有机FRET对，其使用量子点（QD）作为供体，并使用DNA作为连接子，将DNA嵌入染料BOBO-3作为受体。通常，FRET效率随与QD连接的染色DNA数量的增加而增加。但是，通过使用嵌入染料，我们证明通过使用增加的染色染料/ bp比来增加染色至DNA链的染料数量，可以进一步提高固定DNA：QD比下的FRET效率。尽管DNA浓度降低了六倍，我们仍利用染色率的这种灵活性来保持> 0.90的高FRET效率。表征了这种新的QD介导的FRET系统后，我们在细胞环境中使用通过将DNA与商业非病毒基因载体封装在一起而产生的纳米复合物，对该系统进行了测试。使用这种新颖的FRET对，我们能够监测细胞内传递过程中DNA纳米复合物的构型变化和命运，从而为基因传递的机理研究提供了见识。

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