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首页> 外文期刊>Nanotechnology >Transmission electron microscopy for elucidating the impact of silver-based treatments (ionic silver versus nanosilver-containing coating) on the model yeast Saccharomyces cerevisiae
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Transmission electron microscopy for elucidating the impact of silver-based treatments (ionic silver versus nanosilver-containing coating) on the model yeast Saccharomyces cerevisiae

机译：透射电子显微镜用于阐明银基处理（离子银与含纳米银的涂层）对模型酵母酿酒酵母的影响

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摘要

After exposure to ionic silver or nanosilver-containing plasma coating, the same visual aspect of scanning transmission electron microscopy (STEM) images was observed for the model yeast Saccharomyces cerevisiae. The main common feature was the presence of electron-dense nodules all over the cell. However, high resolution TEM (HRTEM), STEM, energy dispersive x-ray microanalysis spectroscopy (EDS) and electron microdiffraction revealed some striking differences. Regarding ionic silver exposure, the formation of electron-dense nodules was related to the Ag~+ reactivity towards sulfur-containing compounds to form clusters with Ag_2S-like structures, together with the production of a few silver nanocrystals, mainly at the cell wall periphery. For nanosilver-based treatment, some sulfur-containing silver clusters preferentially located at the cell wall periphery were detected, together with nodules composed of silver, sulfur and phosphorus all over the cell. In both silver-based treatments, nitrogen and silver signals overlapped, confirming the affinity of silver entities for proteinaceous compounds. Moreover, in the case of nanosilver, interactions of silver with phosphorus-containing subcellular structures were indicated.
机译：暴露于离子银或含纳米银的等离子涂层后，对于模型酵母酿酒酵母观察到相同的扫描透射电子显微镜（STEM）图像的视觉外观。主要的共同特征是整个细胞内都存在电子致密结节。但是，高分辨率TEM（HRTEM），STEM，能量色散X射线微分析光谱（EDS）和电子微衍射显示出一些显着差异。关于离子银的暴露，电子致密结核的形成与对含硫化合物的Ag〜+反应性形成具有Ag_2S样结构的团簇有关，并且主要在细胞壁周围产生了一些银纳米晶体。 。对于基于纳米银的处理，检测到一些优先位于细胞壁外围的含硫银簇，以及整个细胞内由银，硫和磷组成的结核。在两种基于银的处理中，氮和银信号重叠，这证实了银实体对蛋白质化合物的亲和力。此外，在纳米银的情况下，表明了银与含磷亚细胞结构的相互作用。

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