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A hydrogen-bonded electron-tunneling circuit reads the base composition of unmodified DNA

机译:氢键电子隧穿电路读取未修饰的DNA的基本组成

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Using a tunnel junction in which one electrode is guanidinium-functionalized (to trap DNA via hydrogen bonding to the backbone phosphates) and a second electrode which is functionalized with a base (to capture its complementary target on the DNA), current versus distance curves are obtained which yield an accurate measure of the base composition of DNA oligomers. With this long tunneling path, resolution is limited to sequence blocks of about twenty bases or larger, because of the need to form a large-area tunnel junction. A shorter hydrogen-bonded path across bases will be required for DNA sequencing. Nonetheless, these measurements point the way to a new type of nanoscale sensor.
机译:使用一个隧道结,其中一个电极被胍基官能化(以通过氢键合到主链磷酸盐上捕获DNA),第二个电极被碱官能化(以捕获其互补靶标在DNA上),电流-距离曲线为得到的产物可以精确测量DNA寡聚体的基本组成。在这种长的隧道路径中,由于需要形成大面积的隧道结,因此分辨率仅限于约20个碱基或更大的序列块。 DNA测序需要跨越碱基的较短氢键键合路径。尽管如此,这些测量结果为新型纳米级传感器指明了方向。

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