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首页> 外文期刊>Nanotechnology >Synthesis and characterization of polyamidoamine dendrimer-coated multi-walled carbon nanotubes and their application in gene delivery systems
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Synthesis and characterization of polyamidoamine dendrimer-coated multi-walled carbon nanotubes and their application in gene delivery systems

机译：聚酰胺胺树枝状聚合物包覆的多壁碳纳米管的合成，表征及其在基因传递系统中的应用

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摘要

With the aim of improving the amount and delivery efficiency of genes taken by carbon nanotubes into human cancer cells, different generations of polyamidoamine dendrimer modified multi-walled carbon nanotubes (dMNTs) were fabricated, and characterized by high-resolution transmission electron microscopy, atomic force microscopy, x-ray photoelectron spectroscopy, Raman spectroscopy, Fourier transform infrared spectroscopy and thermogravimetric analysis, revealing the presence of dendrimer capped on the surface of carbon nanotubes. The dMNTs fully conjugated with FITC-labeled antisense c-myc oligonucleotides (asODN), those resultant asODN-dMNTs composites were incubated with human breast cancer cell line MCF-7 cells and MDA-MB-435 cells, and liver cancer cell line HepG2 cells, and confirmed to enter into tumor cells within 15 min by laser confocal microscopy. These composites inhibited the cell growth in time- and dose-dependent means, and down-regulated the expression of the c-myc gene and C-Myc protein. Compared with the composites of CNT—NH2—asODN and dendrimer—asODN, no. 5 generation of dendrimer-modified MNT—asODN composites exhibit maximal transfection efficiencies and inhibition effects on tumor cells. The intracellular gene transport and uptake via dMNTs should be generic for the mammalian cell lines. The dMNTs have potentials in applications such as gene or drug delivery for cancer therapy and molecular imaging.
机译：为了提高碳纳米管吸收到人类癌细胞中的基因数量和传递效率，制备了多代聚酰胺型胺树枝状聚合物修饰的多壁碳纳米管（dMNTs），并通过高分辨率透射电子显微镜，原子力进行了表征。显微镜，X射线光电子能谱，拉曼光谱，傅立叶变换红外光谱和热重分析，揭示出树枝状大分子封端在碳纳米管表面。将与FITC标记的反义c-myc寡核苷酸（asODN）完全偶联的dMNTs，那些所得的asODN-dMNTs复合材料与人乳腺癌细胞系MCF-7细胞和MDA-MB-435细胞以及肝癌细胞系HepG2细胞一起孵育，并通过激光共聚焦显微镜确认在15分钟内进入肿瘤细胞。这些复合物以时间和剂量依赖性方式抑制细胞生长，并下调c-myc基因和C-Myc蛋白的表达。与CNT-NH2-asODN和树枝状聚合物-asODN的复合材料相比，没有。第5代树状聚合物修饰的MNT-asODN复合材料表现出最大的转染效率和对肿瘤细胞的抑制作用。经由dMNT的细胞内基因运输和摄取对于哺乳动物细胞系应该是通用的。 dMNT在癌症治疗和分子成像的基因或药物输送等应用中具有潜力。

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