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Metallic nanocone array photonic substrate for high-uniformity surface deposition and optical detection of small molecules

机译：金属纳米锥阵列光子衬底，用于高均匀性表面沉积和小分子的光学检测

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Molecular probe arrays printed on solid surfaces such as DNA, peptide, and protein microarrays are widely used in chemical and biomedical applications especially genomic and proteomic studies(Pollack et al. 1999 Nat.Genet.23 41-6, Houseman et al. 2002 Nat.Biotechnol.20 270-4, Sauer et al. 2005 Nat.Rev.Genet.6 465-76) as well as surface imaging and spectroscopy (Mori et al. 2008 Anal.Biochem.375 223-31, Liu et al. 2006 Nat.Nanotechnol.1 47-52, Liu 2010 IEEE J.Sel.Top.Quantum Electron.16 662-71). Unfortunately the printed molecular spots on solid surfaces often suffer low distribution uniformity due to the lingering 'coffee stain'(Deegan et al. 1997 Nature 389 827-9) problem of molecular accumulations and blotches, especially around the edge of deposition spots caused by solvent evaporation and convection processes. Here we present, without any surface chemistry modification, a unique solid surface of high-aspect-ratio silver-coated silicon nanocone arrays that allows highly uniform molecular deposition and thus subsequent uniform optical imaging and spectroscopic molecular detection. Both fluorescent Rhodamine dye molecules and unlabeled oligopeptides are printed on the metallic nanocone photonic substrate surface as circular spot arrays. In comparison with the printed results on ordinary glass slides and silver-coated glass slides, not only high printing density but uniform molecular distribution in every deposited spot is achieved. The high-uniformity and repeatability of molecular depositions on the 'coffee stain'-free nanocone surface is confirmed by laser scanning fluorescence imaging and surface enhanced Raman imaging experiments. The physical mechanism for the uniform molecular deposition is attributed to the superhydrophobicity and localized pinned liquid-solid-air interface on the silver-coated silicon nanocone surface. The unique surface properties of the presented nanocone surface enabled high-density, high-uniformity probe spotting beneficial for genomic and proteomic microarrays and surface molecular imaging.
机译：印在固体表面上的分子探针阵列，例如DNA，肽和蛋白质微阵列，广泛用于化学和生物医学应用，尤其是基因组和蛋白质组学研究（Pollack等，1999 Nat.Genet.23 41-6，Houseman等，2002 Nat Biotechnol.20 270-4，Sauer等人2005 Nat.Rev.Genet.6 465-76）以及表面成像和光谱学（Mori等人2008 Anal.Biochem.375 223-31，Liu等人。 2006 Nat.Nanotechnol.1 47-52，Liu 2010 IEEE J.Sel.Top.Quantum Electron.16 662-71）。不幸的是，由于挥之不去的“咖啡渍”（Deegan et al。1997 Nature 389 827-9）问题，固体表面上印刷的分子斑点经常遭受较低的分布均匀性，尤其是溶剂引起的沉积斑点边缘附近的分子积累和斑点问题蒸发和对流过程。在这里，我们介绍了没有任何表面化学修饰的高纵横比镀银的硅纳米锥阵列的独特固体表面，该固体表面允许高度均匀的分子沉积，因此可以进行均匀的光学成像和光谱分子检测。荧光若丹明染料分子和未标记的寡肽都以圆点阵列形式印刷在金属纳米锥光子基板表面上。与在普通玻璃载玻片和镀银玻璃载玻片上的印刷结果相比，不仅印刷密度高，而且在每个沉积点的分子分布均匀。激光扫描荧光成像和表面增强拉曼成像实验证实了在无“咖啡渍”的纳米锥表面上分子沉积的高度均匀性和可重复性。均匀的分子沉积的物理机制归因于超疏水性和镀银的硅纳米锥表面上的局部固定的液-固-空气界面。所呈现的纳米锥表面的独特表面特性实现了高密度，高均匀性的探针点样，有利于基因组和蛋白质组学微阵列以及表面分子成像。

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