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首页> 外文期刊>Nanotechnology >Electrochemical impedance measurement of prostate cancer cells using carbon nanotube array electrodes in a microfluidic channel
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Electrochemical impedance measurement of prostate cancer cells using carbon nanotube array electrodes in a microfluidic channel

机译：在微流体通道中使用碳纳米管阵列电极测量前列腺癌细胞的电化学阻抗

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Highly aligned multi-wall carbon nanotubes were synthesized in the shape of towers and embedded into fluidic channels as electrodes for impedance measurement of LNCaP human prostate cancer cells. Tower electrodes up to 8 mm high were grown and easily peeled off a silicon substrate. The nanotube electrodes were then successfully soldered onto patterned printed circuit boards and cast into epoxy under pressure. After polishing the top of the tower electrodes, RF plasma was used to enhance the electrocatalytic effect by removing excess epoxy and activating the open end of the nanotubes. Electrodeposition of Au particles on the plasma-treated tower electrodes was done at a controlled density. Finally, the nanotube electrodes were embedded into a polydimethylsiloxane ( PDMS) channel and electrochemical impedance spectroscopy was carried out with different conditions. Preliminary electrochemical impedance spectroscopy results using deionized water, buffer solution, and LNCaP prostate cancer cells showed that nanotube electrodes can distinguish the different solutions and could be used in future cell-based biosensor development.
机译：以塔状合成高度对齐的多壁碳纳米管，并将其嵌入流体通道中作为电极，用于测量LNCaP人前列腺癌细胞的阻抗。可以生长高达8毫米高的塔形电极，并很容易从硅基板上剥离下来。然后将纳米管电极成功焊接到图案化的印刷电​​路板上，并在压力下铸成环氧树脂。在抛光塔电极的顶部之后，RF等离子体用于通过去除多余的环氧树脂并激活纳米管的开口端来增强电催化效果。在经等离子体处理的塔电极上以受控的密度进行Au粒子的电沉积。最后，将纳米管电极嵌入聚二甲基硅氧烷（PDMS）通道中，并在不同条件下进行电化学阻抗谱分析。使用去离子水，缓冲溶液和LNCaP前列腺癌细胞的初步电化学阻抗谱结果表明，纳米管电极可以区分不同的溶液，可用于未来基于细胞的生物传感器的开发。

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