• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Nanotechnology >MR imaging of Her-2eu protein using magnetic nanopar tides
【24h】

MR imaging of Her-2eu protein using magnetic nanopar tides

机译：使用磁性纳米粒子对Her-2 / neu蛋白进行MR成像

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The aim of this study was to assess whether Her-2eu expressing tumour cells can be detected in vitro as well as in animal tumour models with magnetic resonance imaging at 1.5 T. Magnetic nanoparticles (with relaxivities Rl, R2 of 3.7 + - 0.4 (mM s)~(-1), 277 + - 32 (mM s)~(-1) at 21 deg C, respectively) coupled to anti-Her-2eu antibodies or gamma globulin IgG (high or non-affinity probe, respectively) were used. After incubation of Her-2eu expressing cells (SKBR3) with high or non-affinity probes (20 min), values of R1 = 0.34 + - 0.02 (mM s)~(-1) and R2 = 63.02 + - 30 (mM s)~(-1) were obtained. Electron microscopy and atomic absorption spectrometry examinations verified the presence of relatively high iron levels in cells incubated with the high affinity probe compared to controls. For in vivo MRI, high or non-affinity probes (approx =1.7 mg Fe/animal) were injected into the tail vein of mice (n = 16) bearing SKBR3 tumours. A distinct decrease in the normalized MR signal ratio between tumour and reference area (approximately -17 + - 2 percent) after application of the high affinity probe was observed. In conclusion, in vivo detection of Her-2eu expressing tumours is feasible in a clinical MR scanner by using immunoconjugated magnetic nanoparticles.
机译：这项研究的目的是评估是否可以在体外以及在1.5 T磁共振成像的动物肿瘤模型中检测到表达Her-2 / neu的肿瘤细胞。磁性纳米颗粒（弛豫度R1，R2为3.7 +-0.4 （mM s）〜（-1），分别在21摄氏度时为277 +-32（mM s）〜（-1）与抗Her-2 / neu抗体或伽玛球蛋白IgG（高亲和力或非亲和力）偶联探针）。将Her-2 / neu表达细胞（SKBR3）与高亲和力或非亲和力探针孵育（20分钟）后，R1 = 0.34 +-0.02（mM s）〜（-1）和R2 = 63.02 +-30（获得了mM s）〜（-1）。电子显微镜和原子吸收光谱检查证实，与对照相比，用高亲和力探针孵育的细胞中铁水平较高。对于体内MRI，将高亲和力或非亲和力探针（大约= 1.7 mg Fe /动物）注射入带有SKBR3肿瘤的小鼠尾静脉（n = 16）。观察到在应用高亲和力探针后，肿瘤与参考区域之间的归一化MR信号比率有明显下降（约-17±2％）。总之，通过使用免疫偶联的磁性纳米粒子，在临床MR扫描仪中体内检测Her-2 / neu表达的肿瘤是可行的。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号