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首页> 外文期刊>Nature cell biology >Microtubule-sliding activity of a kinesin-8 promotes spindle assembly and spindle-length control
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Microtubule-sliding activity of a kinesin-8 promotes spindle assembly and spindle-length control

机译：kinesin-8的微管滑动活性可促进主轴组装和主轴长度控制

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Molecular motors play critical roles in the formation of mitotic spindles, either through controlling the stability of individual microtubules, or by crosslinking and sliding microtubule arrays. Kinesin-8 motors are best known for their regulatory roles in controlling microtubule dynamics. They contain microtubule-destabilizing activities, and restrict spindle length in a wide variety of cell types and organisms. Here, we report an antiparallel microtubule-sliding activity of the budding yeast kinesin-8, Kip3. The in vivo importance of this sliding activity was established through the identification of complementary Kip3 mutants that separate the sliding activity and microtubule-destabilizing activity. In conjunction with Cin8, a kinesin-5 family member, the sliding activity of Kip3 promotes bipolar spindle assembly and the maintenance of genome stability. We propose a slide-disassemble model where the sliding and destabilizing activity of Kip3 balance during pre-anaphase. This facilitates normal spindle assembly. However, the destabilizing activity of Kip3 dominates in late anaphase, inhibiting spindle elongation and ultimately promoting spindle disassembly.
机译：分子马达通过控制单个微管的稳定性或通过交联和滑动微管阵列在有丝分裂纺锤体的形成中起关键作用。 Kinesin-8电动机以其在控制微管动力学方面的调节作用而闻名。它们具有破坏微管的活性，并限制了多种细胞类型和生物体中的纺锤体长度。在这里，我们报告了发芽的酵母驱动蛋白8，Kip3的反平行微管滑动活性。这种滑动活性在体内的重要性是通过鉴定互补的Kip3突变体来确定的，该突变体将滑动活性和微管去稳定活性分开。与kinesin-5家族成员Cin8结合，Kip3的滑动活性促进了双极纺锤体的组装和基因组稳定性的维持。我们提出了一个幻灯片拆卸模型，其中Kip3在后期后期保持平衡。这有助于正常的主轴组装。但是，Kip3的去稳定化活性在后期后期占主导地位，抑制了纺锤体的伸长并最终促进了纺锤体的拆卸。

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