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首页> 外文期刊>Biochemistry >Cloning and Structural Analysis of an Indian Little Millet (Panicum sumatrense) Zein-Like Storage Protein:Implications for Molecular Assembly
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Cloning and Structural Analysis of an Indian Little Millet (Panicum sumatrense) Zein-Like Storage Protein:Implications for Molecular Assembly

机译：印度小米（Panicum sumatrense）玉米醇溶蛋白类似的存储蛋白的克隆和结构分析：分子组装的意义。

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摘要

Zeins are prolamin storage proteins that accumulate in kernel endosperm of several cereals.For cloning of genes coding for zein-like proteins that accumulate in enhanced quantities in the filling stages of little millet (Panicum sumatrense Roth.) developing grains,RT-PCR was performed using specific primers.A 750-bp cDNA was directly sequenced and in silico analysis showed high identity degree to alpha-prolamins.This family is composed of zeins from Zea mays,coixins from Coix lachryma-jobi,and alpha-kafirins from Sorghum bicolor.The putative conserved domain of zein-like proteins was identified by primary structure comparisons.Furthermore,threading analyses indicated that the millet zein-like protein forms an anti-parallel alpha-helical hairpin with two opposite surfaces: one hydrophobic and the other hydrophilic that probably could be involved in protein storage assembly.Knowledge about zein-like alpha-prolamins in little millet will lead to cloning and transfer of this gene to other major food crops,such as cereals and legumes,with inferior nutritional quality for monogastric animals.
机译：玉米醇溶蛋白是在几种谷物的内核胚乳中积累的谷醇溶蛋白贮藏蛋白。为克隆编码玉米醇溶蛋白样蛋白的基因，该基因在小米（Panicum sumatrense Roth。）发育的谷粒的灌装阶段大量积累，进行了RT-PCR使用特异性引物直接测序了750 bp cDNA，并在计算机分析中显示了与α-谷醇溶蛋白的高度同一性。通过一级结构比较确定了玉米蛋白样蛋白的假定保守结构域。此外，线程分析表明，小米玉米蛋白样蛋白形成具有两个相反表面的反平行α-螺旋发夹：一个疏水性表面，另一个亲水性表面，可能可能参与蛋白质存储装配。小米中类似玉米蛋白的α-谷醇溶蛋白的知识将导致该基因的克隆并将其转移至其他单胃动物营养质量较差的主要粮食作物，例如谷物和豆类。

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