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Structural Differences between Fusarium Strains Investigated by FT-IR Spectroscopy

机译：FT-IR光谱研究镰刀菌菌株之间的结构差异

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摘要

The structural characteristics of Fusarium have received attention from both pure and applied scientists.Because many genes and physiological mechanisms are involved in the development of a particular structure type,this research is complicated.For revealing the structure of macromolecule in these fungal cells,FT-IR spectroscopy combined with mul tivariate statistical analysis was performed to characterize the structure of protein and polysaccharide of spores and mycelia obtained from different culture medium.The second derivative FT-IR spectra exhibited strain-specific infrared character istics in the protein secondary structure sensitive amide I region.The region between 750 and 950 cm-1 assigned to alpha-and beta-glucans was investigated for studied samples.Principal components analysis(PCA)allowed us to separate mycelia into two clusters according to different growth medium,indicating that spectra of strains may have been greatly affected by cul tivation conditions.
机译：镰刀菌的结构特征已受到纯科学和应用科学家的关注。由于特定基因类型的发展涉及许多基因和生理机制，因此这项研究很复杂。为揭示这些真菌细胞中大分子的结构，FT-红外光谱结合多变量统计分析，表征了不同培养基中孢子和菌丝体的蛋白质和多糖的结构。二阶导数傅立叶变换红外光谱在蛋白质二级结构敏感酰胺I中表现出菌株特异性的红外特征。对研究样品进行了研究，研究了分配给α-和β-葡聚糖的750至950 cm-1之间的区域。主成分分析（PCA）使我们能够根据不同的生长培养基将菌丝体分为两个簇，这表明菌株的光谱可能受栽培条件的影响很大。

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