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首页> 外文期刊>Biochemistry >Influence of Ultraviolet-C on Structure and Function of Synechococcus sp. PCC 7942 Photolyase
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Influence of Ultraviolet-C on Structure and Function of Synechococcus sp. PCC 7942 Photolyase

机译：紫外线-C对Synechococcus sp。结构和功能的影响。 PCC 7942光裂解酶

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摘要

In this work, an over-expressed cyclobutane pyrimidine dimer (CPD) photolyase of Synechococcus sp. PCC 7942 was used to investigate UV-C (ultraviolet irradiation of C-region) influence on photoreactivation. In vivo photoreactivation experiments indicated that the survival rate decreased from 100 to 2.6% when the UV-C flux was increased from 1.1 to 68.5 mu W/cm~2. It seemed that the photolyase was easily inactivated at UV-C intensities >=25.5 mu W/cm~2. Spectrometric analysis indicated that tertiary structure of the photolyase changed evidently when the UV-C fluxes were >=25.5 mu W/cm~2, while the secondary structure was almost unchanged even at 170 mu W/cm~2. Band shift assay indicated that catalytic activity of the photolyase was impaired at fluxes >=25.5 mu W/cm~2, but no significant influence on DNA-binding activity was observed. These results suggest that photoreactivation is efficient at UV-C fluxes <=25.5 mu W/cm~2, but would be impaired by intense UV-C irradiation due to structure changes of the photolyase.
机译：在这项工作中，过度表达的Syechococcus sp。的环丁烷嘧啶二聚体（CPD）光解酶。 PCC 7942用于研究UV-C（C区域的紫外线照射）对光活化的影响。体内光活化实验表明，当UV-C通量从1.1增加到68.5μW/ cm〜2时，存活率从100降低到2.6％。看起来在UV-C强度> =25.5μW/ cm〜2时，光解酶容易失活。光谱分析表明，当UV-C通量大于等于25.5μW/ cm〜2时，光解酶的三级结构发生了明显变化，而二级结构甚至在170μW/ cm〜2时几乎不变。带移分析表明，当通量> = 25.5μW / cm〜2时，光解酶的催化活性受到损害，但未观察到对DNA结合活性的显着影响。这些结果表明，在UV-C通量≤25.5μW/ cm〜2时，光活化是有效的，但是由于光裂解酶的结构变化，强烈的UV-C辐射会损害光活化。

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