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Nutritional and Functional Properties of Aqueous and Hydroalcoholic Extracts from Argentinean Propolis

机译:阿根廷蜂胶水和水醇提取物的营养和功能特性

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Bee propolis is a natural product extensively used as an ingredient in functional foods in amounts that may confer health benefits. The aim of this study was to investigate the nutritional and functional properties (antioxidant activity) of aqueous and ethanolic extracts of propolis samples from Santiago del Estero province, northern Argentina. All propolis extracts contained macronutrients (glucose, fructose, sucrose and proteins), micronutrients (Na, K, Ca, P and Mg) and trace elements (Fe). Spectrophotometric, TLC and HPLC-DAD analyses showed the presence of several phenolic, flavonoid and non-flavonoid compounds, but in all cases the flavonoids prevailed. The PCA of polyphenolic content provided a clear separation of propolis in Group 1 (SE1,2,3,4 and 7) and Group 2 (SE5 and 6) associated with phenolic compound content and collection regions. Two compounds, pinocembrin and chrysin, which could be used as chemical markers of Santiago del Estero propolis, were identified in all samples. Propolis samples extracted with water presented better radical scavenging ability than ethanolic extracts, independent of the antioxidant method (scavenging activity of ABTS~(·+), DPPH~·, HO~· and O_2~(·-) and β-carotene bleaching test). Such results correlated closely with the levels of total phenols and flavonoids in samples. The results justify the use of Argentine propolis as a functional dietary supplement.
机译:蜂胶是一种天然产品,被广泛用作功能性食品的成分,其含量可能带来健康益处。这项研究的目的是调查来自阿根廷北部圣地亚哥德尔埃斯特罗省的蜂胶样品的水和乙醇提取物的营养和功能特性(抗氧化活性)。所有蜂胶提取物均含有大量营养素(葡萄糖,果糖,蔗糖和蛋白质),微量营养素(Na,K,Ca,P和Mg)和微量元素(Fe)。分光光度法,TLC和HPLC-DAD分析表明存在几种酚,类黄酮和非类黄酮化合物,但在所有情况下,类黄酮都占优势。多酚含量的PCA提供了与酚类化合物含量和收集区域相关的第1组(SE1,2,3,4和7)和第2组(SE5和6)中蜂胶的清晰分离。在所有样品中均鉴定出了两种化合物,即松球蛋白和菊花蛋白,可以用作圣地亚哥德尔埃斯特罗蜂胶的化学标记。用水提取的蜂胶样品具有比乙醇提取物更好的自由基清除能力,而与抗氧化剂方法无关(ABTS〜(·+),DPPH〜·,HO〜·和O_2〜(·-)的清除活性以及β-胡萝卜素的漂白测试)。这些结果与样品中总酚和类黄酮的含量密切相关。结果证明使用阿根廷蜂胶作为功能性膳食补充剂是合理的。

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