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首页> 外文期刊>Natural product communications >Conservation and multiplication of encapsulated micro shoots of rauvolfia vomitoria-an endangered medicinal tree: ISSR and RAPD based evaluation of genetic fidelity of converted plantlets
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Conservation and multiplication of encapsulated micro shoots of rauvolfia vomitoria-an endangered medicinal tree: ISSR and RAPD based evaluation of genetic fidelity of converted plantlets

机译：濒危药用树紫杉的包裹微芽的保存和繁殖：基于ISSR和RAPD的转化小植株遗传保真度评估

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摘要

The in vitro grown axillary micro shoots of Rauvolfia vomitoria were encapsulated in alginate beads. Following 6 months of normal storage at 25 ± 2°C the regrowth of encapsulated micro shoots, reached 95.2% within 40 days of incubation on MS medium containing 1.0 mg/L BAP and 0.1 mg/L NAA. Among the responding encapsulated explants 69.6% showed emergence of multiple shoots. The developing shoots showed rhizogenesis in two weeks following their transfer to rooting medium. Healthy plants were established in a glass house with 95% survival. Of the 50 RAPD primers tested, 10 produced 23 clear and reproducible amplicons, with an average of 2.3 bands per primer. Eleven ISSR primers produced a total of 42 bands, with a size range of 0.1-1.9 kb. The number of scorable bands for each primer varied from 2 to 6, with an average of 3.81. The similarity matrix, calculated individually from the results obtained from ISSR and RAPD analysis, showed similarity coefficients ranging from 1.0 for RAPD and 0.85 to 1.0 for ISSR.
机译：将紫薇（Rauvolfia vomitoria）的体外生长腋芽封装在藻酸盐珠粒中。在25±2°C下正常储存6个月后，在含1.0 mg / L BAP和0.1 mg / L NAA的MS培养基上孵育40天之内，封装的微芽的再生长达到95.2％。在响应的封装外植体中，有69.6％出现了多芽。发育中的芽在转移到生根培养基后的两周内显示出发根作用。健康的植物在具有95％存活率的温室中建立。在测试的50种RAPD引物中，有10种产生了23个清晰可重复的扩增子，每个引物平均有2.3条带。共有11条ISSR引物产生了42条带，大小范围为0.1-1.9 kb。每个引物的可计谱带数为2至6，平均为3.81。根据从ISSR和RAPD分析获得的结果分别计算出的相似度矩阵显示，相似度系数的范围从RAPD的1.0到ISSR的0.85到1.0。

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