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A dual-isotope approach to allow conclusive partitioning between three sources

机译：一种双同位素方法，允许在三个源之间进行确定性划分

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Stable isotopes have proved to be a transformative tool; their application to distinguish between two sources in a mixture has been a cornerstone of biogeochemical research. However, quantitatively partitioning systems using two stable isotopes (for example, C-13 and C-12) has been largely limited to only two sources, and systems of interest often have more than two components, with interactive effects. Here we introduce a dual-isotope approach to allow conclusive partitioning between three sources, using only two stable isotopes. We demonstrate this approach by partitioning soil CO2 emissions derived from microbial mineralization of soil organic carbon (SOC), added pyrogenic organic matter (PyOM) and root respiration. We find that SOC mineralization in the presence of roots is 23% higher (P<0.05) when PyOM is also present. Being able to discern three sources with two isotopes will be of great value not only in biogeochemical research, but may also expand hitherto untapped methodologies in diverse fields.
机译：稳定同位素已被证明是一种转化工具。它们在区分混合物中两种来源的应用一直是生物地球化学研究的基石。但是，使用两个稳定同位素（例如C-13和C-12）进行定量分配的系统在很大程度上仅限于两个来源，并且感兴趣的系统通常具有两个以上的成分，并具有交互作用。在这里，我们介绍了一种双同位素方法，允许仅使用两个稳定同位素在三个源之间进行确定性分配。我们通过划分源自土壤有机碳（SOC）的微生物矿化作用的土壤CO2排放，添加的热源有机质（PyOM）和根系呼吸作用来证明这种方法。我们发现，当还存在PyOM时，存在根的SOC矿化度高23％（P <0.05）。能够辨别具有两种同位素的三种来源，不仅在生物地球化学研究中具有重要价值，而且可能会扩展迄今为止在各个领域尚未开发的方法。

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