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首页> 外文期刊>Nature immunology >Distinct classes of human stem cells that differ in proliferative and self-renewal potential
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Distinct classes of human stem cells that differ in proliferative and self-renewal potential

机译：在增殖和自我更新潜能方面不同的人类干细胞的不同类别

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The composition of the human hematopoietic stem cell compartment is poorly understood due to the absence of experimental tools with which to characterize the developmental program of individual stem cells. we report here that human stem cells differ markedly in their repopulation capacity and self-renewal potential, as determined using nonobese diabetic-severe combined immunodeficiency (NOD-SCID) mice transplanted with retrovirally transduced cord blood stem cells, called SCID-repopulating cells (SRCs). Clonal stem cell analysis based on the identification of unique retroviral integration sites within serial bone marrow aspirates showed that repopulation was generally oligoclonal with extensive variability in the lifespan and proliferative capacity of individual SRCs. Most clones contributed to human cell engraftment for several weeks after transplantation and then disappeared but others appeared later and persisted. Further evidence for stem cell heterogeneity was found in the secondary transplantation capacity of SRCs. These data point to the existence of different classes of human stem cells with variable self-renewal potential and short- or long-term repopulating capacity.
机译：由于缺乏用于表征单个干细胞发育程序的实验工具，因此人类造血干细胞区室的组成知之甚少。我们在这里报告说，人类干细胞在其繁殖能力和自我更新潜力方面存在显着差异，这是通过使用非肥胖型糖尿病-重症联合免疫缺陷症（NOD-SCID）小鼠与逆转录病毒转导的脐带血干细胞（称为SCID繁殖细胞（SRC） ）。基于对连续骨髓抽吸物中独特的逆转录病毒整合位点的鉴定的克隆干细胞分析表明，再繁殖通常是寡克隆，单个SRC的寿命和增殖能力具有广泛的变异性。大多数克隆在移植后的几周内促进了人类细胞的移植，然后消失了，但其他克隆出现得更晚并持续存在。干细胞异质性的进一步证据在SRC的二次移植能力中被发现。这些数据表明存在具有可变的自我更新潜力和短期或长期再增殖能力的不同类别的人类干细胞。

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