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首页> 外文期刊>Biological Control: Theory and Application in Pest Management >Ribosomal-sequence-directed selection for endophytic streptomycete strains antagonistic to Ralstonia solanacearum to control tomato bacterial wilt.
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Ribosomal-sequence-directed selection for endophytic streptomycete strains antagonistic to Ralstonia solanacearum to control tomato bacterial wilt.

机译：与番茄青枯病菌拮抗的内生链霉菌菌株的核糖体序列定向选择，以控制番茄的青枯病。

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摘要

The endophytic actinobacterial communities of healthy and wilting tomato plants with different levels of resistance to bacterial wilt were detected by culture-independent and culture-dependent methods to validate the effectiveness of using molecular-profile data of endophytic actinobacterial community structure to rapidly identify and select ecologically important biological control agents from tomato root internals. More than six genera of actinobacteria (Streptomyces, Nesterenkonia, Arthrobacter, Microbacterium, Cellulomonas, and Propionibacterium) were detected in four actinobacterial 16S rRNA gene clone libraries derived from tomato roots. Species similar to Streptomyces virginiae were detected by culture-independent and culture-dependent methods from all the samples. The isolated strains Y30 and E36 similar to S. virginiae showed potent antagonism to Ralstonia solanacearum, producing siderophores, and ACC deaminase activity. The two strains showed significant protection to seedlings from bacterial wilt compared with the control seedlings when inoculated into tomato seedlings in greenhouse although they showed different protecting efficacy. The results showed the validity of using molecular-profile data of endophytic actinobacterial community structure to rapidly identify and select ecologically important actinobacterial species to control tomato bacterial wilt and established the validity of using sequence-directed selection for biocontrol strains from diverse microbial communities.
机译：通过独立于培养和依赖于培养的方法检测健康和枯萎的番茄植物的内生放线菌群落，这些菌株对青枯病具有不同的抗药性，从而验证了利用内生放线菌群落结构的分子谱数据快速识别和生态选择的有效性。番茄根内部的重要生物防治剂。超过六属的放线菌（链霉菌，链霉菌，嗜热杆菌属，微细菌，纤维单胞菌） ”和“丙酸杆菌”在四个源自番茄根的放线菌16S rRNA基因克隆文库中检测到。通过独立于培养物和依赖于培养物的方法从所有样品中检测到与维吉尼亚链霉菌相似的物种。分离的菌株Y30和E36类似于i。弗吉尼亚州对青枯雷尔氏菌有强烈的拮抗作用，产生铁载体，并具有ACC脱氨酶活性。当将两种菌株接种到温室的番茄幼苗中时，与对照幼苗相比，这两种菌株显示出对细菌枯萎的显着保护作用，尽管它们显示出不同的保护功效。结果表明，利用内生放线菌群落结构的分子概况数据快速鉴定和选择具有生态学意义的重要放线菌物种来控制番茄青枯病是有效的，并建立了使用序列定向选择法对来自不同微生物群落的生物防治菌株进行鉴定的有效性。

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