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首页> 外文期刊>Natural product research >Chemical composition, antioxidant activities and protein profiling of different parts of Allamanda cathartica
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Chemical composition, antioxidant activities and protein profiling of different parts of Allamanda cathartica

机译：am兰不同部位的化学成分，抗氧化活性和蛋白质谱

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摘要

The phytochemical screening and protein profiling of Allamanda cathartica was performed. Biochemical analysis revealed that peroxidase (8730 +/- 307 units/g), superoxide dismutase (181 +/- 3.79 units/g), catalase (529 +/- 28.9 units/g), protease (3598 +/- 79.8 units/g), total phenolic contents (19,344 +/- 657 mu M/g), beta-esterases (342 +/- 46.5 units/g) and the total oxidant status were highest in the roots as compared to other plant parts. However, total soluble proteins (128 +/- 1.54 mg/g), lycopene (5.70 +/- 0.61 mg/g), chlorophyll a (161 +/- 24.9 mg/g), total chlorophyll content (267 +/- 34.3mg/g) and total carotenoid content (12.4 +/- 1.71 mg/g) were found to be highest in leaves. Moreover, total antioxidant capacity (5.43 +/- 0.29 mu M/g) detected by using ABTS method and a-esterase (714.580 +/- 23.6 units/g) were highest in shoots. The protein profiling was performed using SDS-PAGE. In leaves, 13 peptides with molecular weight (M. wt.) from 27 to 168 kDa were detected while in shoots 10 peptides with M. wt. from 30 to 95 kDa were resolved. Similarly, in roots, 10 peptides of 30-880 kDa and in flower seven peptides of 30-88 kDa were detected.
机译：进行了黄蔓的植物化学筛选和蛋白质谱分析。生化分析显示过氧化物酶（8730 +/- 307单位/ g），超氧化物歧化酶（181 +/- 3.79单位/ g），过氧化氢酶（529 +/- 28.9单位/ g），蛋白酶（3598 +/- 79.8单位/与其他植物部位相比，根中的总酚含量（19,344 +/- 657μM / g），β-酯酶（342 +/- 46.5单位/ g）和总氧化剂状态最高。但是，总可溶性蛋白（128 +/- 1.54 mg / g），番茄红素（5.70 +/- 0.61 mg / g），叶绿素a（161 +/- 24.9 mg / g），总叶绿素含量（267 +/- 34.3）毫克/克）和总类胡萝卜素含量（12.4 +/- 1.71毫克/克）在叶中最高。此外，用ABTS法检测到的总抗氧化能力（5.43 +/- 0.29μM/ g）和α-酯酶（714.580 +/- 23.6单位/ g）在芽中最高。使用SDS-PAGE进行蛋白质谱分析。在叶片中，检测到13种分子量（M. wt。）从27至168 kDa的肽，而在芽中则检测到10种具有M. wt。从30到95 kDa解析。类似地，在根中检测到10个30-880 kDa的肽，在花中检测到7个30-88 kDa的肽。

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