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首页> 外文期刊>Biological Control: Theory and Application in Pest Management >Microbial biological control potential of three strains of Beauveria bassiana s. l. against greenhouse shore fly Scatella tenuicosta: assessment of virulence, mass production capacity, and effects on shore fly reproduction.
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Microbial biological control potential of three strains of Beauveria bassiana s. l. against greenhouse shore fly Scatella tenuicosta: assessment of virulence, mass production capacity, and effects on shore fly reproduction.

机译:3种白僵菌的微生物防治潜力。 l。针对温室蝇蝇Scatella tenuicosta:评估毒力,大规模生产能力以及对蝇蝇繁殖的影响。

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摘要

The microbial biological control potential of three strains of Beauveria bassiana sensu lato originally isolated from the shore fly Scatella tenuicosta (Diptera: Ephydridae) was assessed in a series of laboratory bioassays. Comparisons were made to two commercially-available strains of B. bassiana. Two of the shore fly strains proved 27-67 times more virulent than the commercial strains in terms of LC50 (14-17 vs. 458-942 conidia/mm2) and killed shore flies more rapidly. B. bassiana s. l. strain ST1 exhibited a mass production capacity comparable to the commercial B. bassiana stain GHA, producing 2.8x1012 conidia/kg barley-based solid substrate in ventilated mushroom spawn bags. The shore fly strains of Beauveria sporulated on a higher percentage of killed adult shore flies and produced substantially greater numbers of conidia per cadaver than the commercial strains, indicating that these pathogens are well adapted to this host. Female shore flies treated with strain ST1 survived for only 5 days, with longevity being reduced by 8-10 days compared to control insects. This reduction in survival had a large impact on total lifetime egg production, reducing it by 78-88%, depending on the time of treatment relative to the pre-oviposition period. However, fungal growth within infected female shore flies had no effect on egg production or egg viability until the day before the flies succumbed to mycosis (day 4 post-inoculation). As a consequence, the intrinsic rate of shore fly population increase and population doubling time were little affected by fungal infection (0.4357 vs. 0.4152 and 1.6 vs. 1.7 days for control vs. Beauveria-treated populations, respectively). These findings underscore the challenges involved with use of slow-acting pathogens for control of highly fecund greenhouse pests and the fundamental necessity of integrating these agents into integrated pest management systems.
机译:在一系列实验室生物测定中,评估了最初从岸蝇天蛾科小翅藻(Diptera:Ephydridae)中分离的三种球孢白僵菌菌株的微生物生物学控制潜力。与两种商业可得的球孢白僵菌菌株进行了比较。就LC 50 而言,其中两个岸蝇菌株的毒力比市售菌株高27-67倍(14-17 vs. 458-942分生孢子/ mm 2 )并杀死了海岸飞得更快。巴氏芽孢杆菌l。菌株ST1的批量生产能力可与商业的球孢杆菌(B. bassiana)染色剂GHA相媲美,在通风的蘑菇产卵袋中产生2.8x10 12 分生孢子/千克大麦基固体基质。与商业菌株相比,白僵菌的海岸蝇菌株在较高百分比的被杀死的成年海岸蝇上形成孢子,并且每个尸体产生的分生孢子数量要多得多,这表明这些病原体非常适合这种寄主。用ST1菌株处理的雌性滨蝇仅存活5天,与对照昆虫相比,寿命降低了8-10天。存活率的降低对终生卵的总产量有很大影响,取决于相对于排卵前的治疗时间,其降低了78-88%。但是,直到雌蝇死于霉菌病的前一天(接种后第4天),被感染的雌性岸蝇中的真菌生长对卵的产生或卵的存活率没有影响。结果,真菌感染对岸蝇种群的内在增长率和种群倍增时间几乎没有影响(对照和白僵菌处理种群分别为0.4357 vs. 0.4152和1.6 vs. 1.7天)。这些发现强调了使用慢效病原体控制高度繁殖力温室害虫所涉及的挑战,以及将这些药剂整合到病虫害综合治理系统中的基本必要性。

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