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首页> 外文期刊>Biochemistry >POLYELECTROLYTE COUNTERION CONDENSATION THEORY EXPLAINS DIFFERENTIAL SCANNING CALORIMETRY STUDIES OF SALT-INDUCED CONDENSATION OF CHICKEN ERYTHROCYTE CHROMATIN
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POLYELECTROLYTE COUNTERION CONDENSATION THEORY EXPLAINS DIFFERENTIAL SCANNING CALORIMETRY STUDIES OF SALT-INDUCED CONDENSATION OF CHICKEN ERYTHROCYTE CHROMATIN

机译：聚电解质抗衡析理论解释了盐诱导的鸡红细胞染色质冷凝的差示扫描量热法

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摘要

The salt-induced chromatin condensation in chicken erythrocyte nuclei is studied by differential scanning calorimetry (DSC). The degree of chromatin condensation is measured for condensation induced by monovalent, divalent, trivalent, or tetravalent cations and by a mixture of sodium and magnesium. These last two cations show an evident competition effect. Salt-induced chromatin condensation is shown to be an entropy-driven process. A simple model of chromatin based on the polyelectrolyte counterion condensation theory is used in order to compute the charge neutralized by the cations in each chromatin domain. The degree of chromatin condensation is shown to be related to the weighed sum of the square of the phosphate charge of each domain. The model predicts the salt and the chromatin concentration dependence of the condensation and the effect of H1 removal.
机译：用差示扫描量热法（DSC）研究了盐诱导的鸡红细胞核中的染色质浓缩。对于由一价，二价，三价或四价阳离子以及钠和镁的混合物引起的缩合，测量染色质的缩合度。后两个阳离子显示出明显的竞争效应。盐诱导的染色质凝结是一个熵驱动的过程。为了计算每个染色质域中的阳离子所中和的电荷，使用了基于聚电解质抗衡离子缩合理论的简单染色质模型。染色质的缩合度显示为与每个区域的磷酸盐电荷平方的加权总和相关。该模型预测了缩合盐和染色质浓度的依赖性以及去除H1的影响。

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