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首页> 外文期刊>Neurourology and urodynamics. >Maturation of stretch-induced contractile activity and its muscarinic regulation in isolated whole bladder strips from rat.
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Maturation of stretch-induced contractile activity and its muscarinic regulation in isolated whole bladder strips from rat.

机译：拉伸诱导的收缩活性的成熟及其在大鼠分离的全膀胱条中的毒蕈碱调节。

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AIM: Besides the establishment of neural reflex pathways, developmental changes in local bladder properties probably also contribute to the onset of mature voiding reflexes. Here we explored the behavior of stretch-induced contractile activity (SIC) and its muscarinic regulation in neonatal and adult rat bladders. METHODS: SIC was studied in bladder strips from D0, D7, D28 rat pups and adult rats (15 weeks). The responses to a non-selective [Carbachol (CA), 10(-8.5)-10(-6) M] and an M(2)-selective muscarinic agonist [arecaidine but-2-ynyl ester tosylate (ABET), 10(-9.5)-10(-7) M] were studied. The expression of M(2) and M(3) mRNA was investigated using quantitative PCR in whole bladders. The response of SIC to KCl (50 mM) and to the non-adrenergic non-cholinergic (NANC) drugs Substance P (1 microM) and alpha,beta-Methyleneadenosine 5'-triphosphate lithium salt (MATP) (1 microM) were also studied. RESULTS: We found evidence for an enhanced response to the muscarinic agonists CA and ABET in neonatal bladders. This might be due to the onset of a direct contractile role for M(2), given the moderate M(2)-properties of ABET and the absence of ABET-effects on adult bladder strips. Further data showed an increased expression of both M(2) and M(3) at the mRNA level and a changed response of SIC to NANC drugs in neonatal bladder. CONCLUSIONS: This study reveals a changed response of SIC to muscarinic and NANC drugs in neonatal rat bladder together with changes at the muscarinic mRNA level, which might all contribute to a better insight in the role of SIC in the onset of mature voiding.
机译：目的：除了建立神经反射通路外，局部膀胱特性的发育变化也可能有助于成熟的排尿反射。在这里，我们探讨了在新生和成年大鼠膀胱中拉伸诱导的收缩活动（SIC）的行为及其毒蕈碱调节。方法：在D0，D7，D28大鼠幼崽和成年大鼠的膀胱试纸上研究了SIC（15周）。对非选择性[卡巴胆碱（CA），10（-8.5）-10（-6）M]和M（2）-选择性毒蕈碱激动剂[槟榔碱丁-2-炔基酯甲苯磺酸盐（ABET），10研究了（-9.5）-10（-7）M]。 M（2）和M（3）mRNA的表达进行了定量PCR在整个膀胱。 SIC对KCl（50 mM）和对非肾上腺素非胆碱能（NANC）药物的反应P物质（1 microM）和α，β-亚甲基腺苷5'-三磷酸锂盐（MATP）（1 microM）也是。研究。结果：我们发现证据表明新生儿膀胱中毒蕈碱激动剂CA和ABET的反应增强。考虑到ABET具有中等M（2）-性质，并且在成人膀胱带上没有ABET效应，这可能是由于M（2）直接收缩作用的开始。进一步的数据显示M（2）和M（3）在mRNA水平上的表达均增加，并且SIC对新生膀胱中NANC药物的反应发生了改变。结论：这项研究揭示了新生鼠膀胱中SIC对毒蕈碱和NANC药物的反应发生了变化，同时毒蕈碱mRNA水平也发生了变化，这可能有助于更好地了解SIC在成熟排尿中的作用。

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