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首页> 外文期刊>Nematologia Mediterranea >Nematicidal activity of Strychnos nuxvomica leaf and its constituents against the root-knot nematode, Meloidogyne incognita.
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Nematicidal activity of Strychnos nuxvomica leaf and its constituents against the root-knot nematode, Meloidogyne incognita.

机译：马兜铃叶片及其成分对根结线虫Meloidogyne incognita的杀线虫活性。

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摘要

Studies were undertaken on the nematicidal activity of Strychnos nuxvomica leaf extracts and its constituents on the second stage juveniles of the root-knot nematode, Meloidogyne incognita. Aqueous leaf extracts at 2% concentration caused 100% mortality of second stage juveniles of the nematode. The biological activity of the known phytochemicals from S. nuxvomica, evaluated by in silico analysis through prediction of activity spectra for substances (PASS) software, indicated that seven phytochemicals were expected to show nematicidal activity at various levels. In silico studies showed that the values of Pa (probability to be active) for brucine and strychnine were 0.986 and 0.785, respectively. Bioassay-guided fractionation of the leaf extract of S. nuxvomica indicated that the active fractions contained brucine and strychnine as major components. The nematicidal activity of brucine and strychnine was validated through in vitro bioassays and LD50 values were determined to be 665 ppm and 833 ppm, respectively.
机译：研究了马兜铃叶片提取物及其对根结线虫第二代幼虫Meloidogyne incognita的杀虫活性。浓度为2％的叶子水提物导致线虫第二阶段幼虫的死亡率为100％。通过预测物质活性谱（PASS）软件进行的计算机分析，通过计算机分析分析，评估了来自新链霉菌的已知植物化学物质的生物活性，表明预期有七种植物化学物质在各个水平上均显示杀线虫活性。在计算机上进行的研究表明，苯丙氨酸和士的宁的Pa（有活性的可能性）的值分别为0.986和0.785。生物测定法指导的链霉葡萄球菌叶提取物分级分离表明，活性级分含有布鲁辛和士的宁为主要成分。通过体外生物测定法验证了苯丙氨酸和士的宁的杀线虫活性，LD 50 值分别确定为665 ppm和833 ppm。

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