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首页> 外文期刊>Neurochemistry International: The International Journal for the Rapid Publication of Critical Reviews, Preliminary and Original Research Communications in Neurochemistry >Melatonin biosynthesis in photoreceptor-enriched chick retinal cell cultures: role of cyclic AMP in the K(+)-evoked, Ca(2+)-dependent induction of serotonin N-acetyltransferase activity.
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Melatonin biosynthesis in photoreceptor-enriched chick retinal cell cultures: role of cyclic AMP in the K(+)-evoked, Ca(2+)-dependent induction of serotonin N-acetyltransferase activity.

机译：褪黑素生物合成的光感受器富集的鸡视网膜细胞培养物中：环AMP在K（+）诱发，Ca（2+）依赖的血清素N-乙酰转移酶活性的诱导中的作用。

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摘要

The roles of cyclic AMP and calcium in the regulation of serotonin N-acetyltransferase (NAT) activity were studied in low density monolayer cultures of chick retinal photoreceptors and neurons. Photoreceptor-enriched retinal cell cultures were prepared from embryonic day 6 retinas and cultured for 6 days. NAT activity in these cultures could be induced by treatment with cyclic AMP protagonists, 8Br-cyclic AMP, forskolin, and 3-isobutyl-1-methylxanthine (IBMX), or by treatment with depolarizing concentrations of extracellular K+. The stimulatory effect of K+, which involves Ca2+ influx through dihydropyridine-sensitive channels, was mediated at least in part by cyclic AMP, as indicated by the following observations. Depolarizing concentrations of K+ stimulated the formation of cyclic AMP, and the stimulatory effects of K+ on both cyclic AMP formation and on NAT activity were synergistically potentiated by the cyclic nucleotide phosphodiesterase inhibitor 3-isobutyl-1-methylxanthine (IBMX). MDL 12,330A, a putative adenylate cyclase inhibitor, inhibited K(+)-evoked cyclic AMP accumulation and induction of NAT activity over the identical concentration range. In contrast, MDL 12,300A failed to inhibit the induction of NAT elicited by 8Br-cyclic AMP. H-89, an inhibitor of cyclic AMP-dependent protein kinase, antagonized the induction of NAT activity by either forskolin or K+ with equal potency for both stimuli. These results suggest that cyclic AMP plays an essential role in the induction of NAT activity that occurs as a consequence of membrane depolarization. Cyclic AMP and Ca2+ may also interact at a step distal to adenylate cyclase.(ABSTRACT TRUNCATED AT 250 WORDS)
机译：在雏鸡视网膜感光细胞和神经元的低密度单层培养物中研究了环状AMP和钙在5-羟色胺N-乙酰转移酶（NAT）活性调节中的作用。从胚胎第6天视网膜制备富含光感受器的视网膜细胞培养物，并培养6天。这些培养物中的NAT活性可以通过用环AMP激动剂，8Br-环AMP，福司可林和3-异丁基-1-甲基黄嘌呤（IBMX）处理或用去极化浓度的细胞外K +处理来诱导。如以下观察结果所示，涉及通过二氢吡啶敏感性通道的Ca 2+流入的K +的刺激作用至少部分地由环状AMP介导。 K +的去极化浓度刺激了环状AMP的形成，并且环状核苷酸磷酸二酯酶抑制剂3-异丁基-1-甲基黄嘌呤（IBMX）协同增强了K +对环状AMP形成和NAT活性的刺激作用。 MDL 12,330A，一种假定的腺苷酸环化酶抑制剂，在相同的浓度范围内抑制K（+）引起的环AMP积累和NAT活性的诱导。相反，MDL 12,300A无法抑制8Br-环AMP诱导的NAT诱导。 H-89是环状AMP依赖性蛋白激酶的抑制剂，它与毛喉素或K +拮抗NAT活性的诱导作用，对两种刺激均具有相同的效力。这些结果表明，环状AMP在诱导NAT活性中起着至关重要的作用，而NAT活性是由于膜去极化而产生的。环状AMP和Ca2 +也可能在腺苷酸环化酶远端的一个步骤处相互作用（摘要以250字截短）

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