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首页> 外文期刊>Neurochemistry International: The International Journal for the Rapid Publication of Critical Reviews, Preliminary and Original Research Communications in Neurochemistry >Neuronal localization of metallothioneins in rat and human spinal cord.
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Neuronal localization of metallothioneins in rat and human spinal cord.

机译：金属硫蛋白在大鼠和人脊髓中的神经元定位。

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摘要

The immunocytochemical localization of the metallothioneins (MT), a family of low molecular weight, heavy metal binding proteins, was investigated in rat and human spinal cord using two distinct antibodies. Polyclonal antibodies were raised to the peptide (SCKCKECKCTS), an epitope common to all human MT isoforms. The monoclonal antibody, E9 which recognizes a different epitope of the MT protein, was also used. Similar results were obtained with either antibody. Both antibodies recognized authentic MT from rabbit liver and horse kidney on Western blots. Immunoreactivity was localized primarily in the cytoplasm of motoneurons in the anterior horn of cervical and lumbar cord. However nuclear staining was present in some motoneurons in both rat and human cord. Intense nuclear, as well as cytoplasmic staining was seen in astrocytes. Capillary endothelia, ependymal cells, arachnoid and pia were also positive for MT.
机译：低水平，重金属结合蛋白家族的金属硫蛋白（MT）的免疫细胞化学定位使用两种不同的抗体在大鼠和人的脊髓中进行了研究。产生针对该肽（SCKCKECKCTS）的多克隆抗体，SCKCKECKCTS是所有人类MT同工型共有的表位。还使用了识别MT蛋白不同表位的单克隆抗体E9。两种抗体都获得了相似的结果。两种抗体在Western印迹上均能识别来自兔肝和马肾的真实MT。免疫反应性主要定位在子宫颈和腰椎前角运动神经元的细胞质中。然而，大鼠和人类脐带的某些运动神经元中都存在核染色。在星形胶质细胞中观察到强烈的核以及细胞质染色。毛细血管内皮，室管膜细胞，蛛网膜和pia也对MT呈阳性。

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