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首页> 外文期刊>Neurochemistry International: The International Journal for the Rapid Publication of Critical Reviews, Preliminary and Original Research Communications in Neurochemistry >Intracellular metabolic compartmentation assessed by 13C magnetic resonance spectroscopy.
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Intracellular metabolic compartmentation assessed by 13C magnetic resonance spectroscopy.

机译：通过13 C磁共振波谱评估细胞内代谢区室。

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Our understanding of the brain has developed from the theory that it is one continuous cell to the knowledge that there are many brain cells originally termed neurons and, furthermore to the discovery of glial cells and their multiple functions. Thus, an increasing complexity was unraveled and we have not reached a complete understanding of the phenomenon which comprises the compartmentation of metabolic pathways and metabolites. This is an important principle needed to fully understand the metabolic processes of the brain. At the cellular level this concept is well established whereas intracellular compartmentation has yet to be explored. Using magnetic resonance spectroscopy (MRS) for analysis of isotopomer composition combined with quantification of amino acid contents it is possible to construct models that describe intracellular compartmentation. Results of studies of cultures of astrocytes and neurons incubated in media containing [U- 13C]glutamate in the presence or absence of thiopental may be used to propose an intracellular three compartment model of mitochondrial function. Due to the experimental paradigm only certain aspects of metabolism can be described. The present model consists of compartments assigned to CO(2) production, glutamate synthesis from ketoglutarate and finally synthesis of a four-carbon metabolite which is shuttled between compartments. It is likely that metabolism may be far more complex than this and we are only beginning to glimpse some aspects of compartmentation at the cellular level.
机译：我们对大脑的理解是从“一个连续的细胞”这一理论发展到知道有许多最初称为神经元的脑细胞，再到发现神经胶质细胞及其多种功能。因此，人们已经阐明了日益增加的复杂性，并且我们还没有完全理解包括代谢途径和代谢产物在内的现象。这是充分理解大脑代谢过程所需的重要原理。在细胞水平上，这一概念已得到很好的确立，而细胞内区室化尚待探索。使用磁共振波谱法（MRS）分析同位异构体组成并结合氨基酸含量的定量，可以构建描述细胞内区室的模型。在存在或不存在硫喷妥钠的条件下，在含有[U-13C]谷氨酸的培养基中孵育的星形胶质细胞和神经元培养物的研究结果可用于提出线粒体功能的细胞内三室模型。由于实验范例，只能描述新陈代谢的某些方面。本模型由分配给CO（2）生产的隔室组成，由酮戊二酸酯合成谷氨酸，最后合成了在各隔室之间穿梭的四碳代谢物。代谢可能比这复杂得多，我们才刚刚开始了解细胞水平上的区室化的某些方面。

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