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首页> 外文期刊>Neurochemistry International: The International Journal for the Rapid Publication of Critical Reviews, Preliminary and Original Research Communications in Neurochemistry >Intracellular oxidative activity and respiratory burst of leukocytes isolated from multiple sclerosis patients.
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Intracellular oxidative activity and respiratory burst of leukocytes isolated from multiple sclerosis patients.

机译：从多发性硬化症患者中分离出的白细胞的细胞内氧化活性和呼吸爆发。

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Oxidative damage induced by free radicals and reactive oxygen species (ROS) have been suggested to play an important role in the development of autoimmune diseases such as multiple sclerosis (MS) disease and it has been hypothesised that oxidative injury could mediate demyelination and axonal injury in MS subjects. In our study, we compared intracellular oxidative activity and the respiratory burst activity in MS patients (n=20) and healthy controls (n=15) using leukocytes as cellular model. At this purpose, intracellular ROS levels were evaluated by fluorometric assay using the 2'-7'-dichlorodihydrofluorescin diacetate probe (H(2)DCFDA) in untreated or in leukocytes stimulated with phorbol-12-myristate-13-acetate (PMA). Our results demonstrate that the intracellular spontaneous ROS production in leukocytes from MS patients was higher with respect to cells from control subjects (p<0.001). PMA addition induced a higher formation of ROS both in leukocytes from MS patients and controls (p<0.001). The PMA-induced production of ROS was significantly higher in leukocytes from MS with respect to controls (p<0.001). Significant positive correlations were established between intracellular spontaneous or PMA-induced production of ROS in leukocytes isolated from MS patients and the clinical parameters used to evaluate disease disability such as expanded disability status scale (EDSS), brain lesions evaluated by MRI and visual evoked potential (VEP) (p<0.001). In conclusion, our results demonstrate higher levels of intracellular ROS in untreated or in PMA-treated leukocytes isolated from MS patients with respect to healthy subjects confirming the role of oxidative stress in multiple sclerosis.
机译：自由基和活性氧（ROS）引起的氧化损伤已被证明在自身免疫性疾病（如多发性硬化症（MS）疾病）的发展中起重要作用，并且据推测，氧化损伤可介导脱髓鞘和轴突损伤。 MS科目。在我们的研究中，我们以白细胞为细胞模型比较了MS患者（n = 20）和健康对照（n = 15）的细胞内氧化活性和呼吸爆发活性。为此，使用2'-7'-dichlorodihydrofluorescin diacetate探针（H（2）DCFDA），通过荧光测定法评估细胞内ROS的水平，方法是用未经处理或经phorbol-12-肉豆蔻酸酯-13-乙酸酯（PMA）刺激的白细胞。我们的结果表明，与对照组相比，MS患者白细胞的细胞内自发ROS产量更高（p <0.001）。 PMA的添加在MS患者和对照组的白细胞中均诱导了较高的ROS形成（p <0.001）。与对照相比，PMA诱导的PMA诱导的ROS产生在MS的白细胞中明显更高（p <0.001）。在从MS患者中分离出的白细胞中细胞内自发或PMA诱导的ROS产生与用于评估疾病残疾的临床参数之间建立了显着的正相关性，所述临床参数例如是扩大残疾状态量表（EDSS），通过MRI评估的脑损伤和视觉诱发电位（ VEP）（p <0.001）。总之，我们的结果表明，相对于健康受试者，从MS患者中分离出的未经治疗或PMA治疗的白细胞中细胞内ROS的水平较高，证实了氧化应激在多发性硬化症中的作用。

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