...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Neurochemistry International: The International Journal for the Rapid Publication of Critical Reviews, Preliminary and Original Research Communications in Neurochemistry >Copper accumulation by cultured astrocytes.
【24h】

Copper accumulation by cultured astrocytes.

机译：培养的星形胶质细胞积累铜。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

To study copper transport in brain astrocytes, we have used astrocyte-rich primary cultures as model system. Cells in these cultures contained a basal copper content of 1.1+/-0.4 nmol per mg protein. The cellular copper content increased strongly after application of copper chloride in a time and concentration-dependent manner. Analysis of the linear copper accumulation during the first 5 min of copper exposure revealed that cultured astrocytes accumulated copper with saturable kinetics with apparent K(M)- and V(max)-values of 9.4+/-1.8 microM and 0.76+/-0.13 nmol/(min x mg protein), respectively. In contrast, incubation of astrocytes with copper in the presence of ascorbate caused a linear increase of the copper accumulation rates for copper concentrations of up to 30 microM. In addition, copper accumulation was strongly inhibited by the presence of an excess of zinc or of various other divalent metal ions. The presence of mRNA and of immunoreactivity of the copper transport protein Ctr1 in astrocyte cultures suggests that Ctr1 contributes to the observed copper accumulation. However, since some characteristics of the observed copper accumulation are not consistent with Ctr1-mediated copper transport, additional Ctr1-independent mechanism(s) are likely to be involved in astrocytic copper accumulation.
机译：为了研究脑星形胶质细胞中铜的转运，我们使用了富含星形胶质细胞的原代培养物作为模型系统。这些培养物中的细胞的基础铜含量为每毫克蛋白质1.1 +/- 0.4nmol。氯化铜以时间和浓度依赖性方式施加后，蜂窝状铜含量急剧增加。铜暴露的前5分钟内线性铜积累的分析表明，培养的星形胶质细胞以饱和动力学积累铜，其表观K（M）-和V（max）值为9.4 +/- 1.8 microM和0.76 +/- 0.13 nmol /（最小x毫克蛋白质）。相比之下，星形胶质细胞与铜在抗坏血酸存在下温育会导致铜浓度高达30 microM时铜累积速率线性增加。另外，过量的锌或各种其他二价金属离子的存在强烈抑制了铜的积累。星形胶质细胞培养物中铜转运蛋白Ctr1的mRNA和免疫反应性的存在表明Ctr1有助于观察到的铜积累。但是，由于观察到的铜积累的某些特征与Ctr1介导的铜迁移不一致，因此星形胶质铜的积累可能涉及其他独立于Ctr1的机制。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号