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首页> 外文期刊>Neurochemistry International: The International Journal for the Rapid Publication of Critical Reviews, Preliminary and Original Research Communications in Neurochemistry >Long-term dietary restriction modulates the level of presynaptic proteins in the cortex and hippocampus of the aging rat.
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Long-term dietary restriction modulates the level of presynaptic proteins in the cortex and hippocampus of the aging rat.

机译：长期的饮食限制会调节衰老大鼠皮质和海马中突触前蛋白的水平。

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摘要

Brain aging is related to the numerous structural and functional changes including decreased synaptic plasticity. The beneficial effects of dietary restriction (DR) are well known but insufficiently investigated at the level of plasticity-related markers. Therefore, the aim of this study was to examine the expression profiles of proteins structurally and functionally related to synapses-growth-associated protein 43 (GAP-43), synaptophysin (SPH) and alpha-synuclein (alpha-Syn), in the course of aging and in response to long-term DR. The mRNA and protein levels of three presynaptic proteins were assessed by Real Time RT-PCR and Western blotting in the cortex and hippocampus of young (6-month-old), middle-aged (12-month-old), aged (18-month-old) and old (24-month-old) male Wistar rats fed ad libitum and exposed to DR starting from 6 months of age. We observed that long-term DR modulated age-related transcriptional changes by maintaining stable mRNAs levels in the cortex. No major age-related changes of the protein levels were observed in the cortex, while the specific temporal decline was detected in the hippocampus for all three proteins. The SPH levels were decreased across lifespan (0.8-, 0.8- and 0.6-fold change at 12, 18 and 24 months), while the significant decrease of GAP-43 and alpha-Syn protein was detected at 24 months of age (0.6- and 0.7-fold decrease, respectively). Long-term DR eliminated this decline by increasing GAP-43, SPH and alpha-Syn protein levels (1.7-, 1.7- and 1.6-fold, respectively) thus reverting protein levels to the values measured in 6-month-old animals.Specific pattern of changes observed in the hippocampus identifies this structure as more vulnerable to the processes of aging and with a more pronounced response to the DR effects. The observed DR-induced stabilization of the levels of three presynaptic proteins indicates the beneficial effect of DR on age-related decline in the capacity for synaptic plasticity.
机译：脑衰老与许多结构和功能变化有关，包括突触可塑性下降。饮食限制（DR）的有益作用是众所周知的，但在可塑性相关标记物水平上的研究不足。因此，本研究的目的是在此过程中检查与突触生长相关蛋白43（GAP-43），突触素（SPH）和α-突触核蛋白（α-Syn）结构和功能相关的蛋白质的表达谱。老化和对长期DR的反应。通过实时RT-PCR和Western印迹法评估了年龄在18岁至18岁之间的年轻人（6个月大），中年（12个月大）的皮层和海马中三种突触前蛋白的mRNA和蛋白水平。 1个月大和24个月大的雄性Wistar大鼠从6个月大开始随意喂食并暴露于DR。我们观察到，长期DR通过维持皮层中稳定的mRNA水平来调节年龄相关的转录变化。在皮质中未观察到蛋白质水平的主要年龄相关变化，而所有三种蛋白质在海马中均检测到特定的时间下降。 SPH水平在整个生命周期中均下降（在12、18和24个月时分别变化0.8-，0.8-和0.6倍），而在24个月大时检测到GAP-43和α-Syn蛋白显着下降（0.6-和分别降低0.7倍）。长期DR通过增加GAP-43，SPH和α-Syn蛋白水平（分别为1.7、1.7和1.6倍）消除了这种下降，从而将蛋白水平恢复为6个月大动物的测量值。在海马体中观察到的变化模式表明该结构更易于老化，并且对DR效应的反应更为明显。观察到的DR诱导的三种突触前蛋白水平的稳定表明DR对与年龄有关的突触可塑性下降的有益作用。

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