• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Neurochemistry International: The International Journal for the Rapid Publication of Critical Reviews, Preliminary and Original Research Communications in Neurochemistry >Glutamate regulates eEF1A phosphorylation and ribosomal transit time in Bergmann glial cells.
【24h】

Glutamate regulates eEF1A phosphorylation and ribosomal transit time in Bergmann glial cells.

机译：谷氨酸调节Bergmann神经胶质细胞中的eEF1A磷酸化和核糖体传递时间。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Glutamate, the major excitatory transmitter in the vertebrate brain, is involved in neuronal development and synaptic plasticity. Glutamatergic stimulation leads to differential gene expression patterns in neuronal and glial cells. A glutamate-dependent transcriptional control has been established for several genes. However, much less is known about the molecular events that modify the translational machinery upon exposure to this neurotransmitter. In a glial model of cerebellar cultured Bergmann cells, glutamate induces a biphasic effect on [(35)S]-methionine incorporation into proteins that suggests that the elongation phase of protein biosynthesis is the target for regulation. Indeed, after a 15 min exposure to glutamate a transient increase in elongation factor 2 phosphorylation has been reported, an effect mediated through the activation of the elongation factor 2 kinase. In this contribution, we sought to characterize the phosphorylation status of the eukaryotic elongation factor 1A (eEF1A) and the ribosomal transit time under glutamate exposure. A dose-dependent increase in eEF1A phosphorylation was found after a 60 min glutamate treatment; this phenomenon is Ca(2+)/CaM dependent, blocked with Src and phosphatidyl-inositol 3-kinase inhibitors and with rapamicyn. Concomitantly, the ribosomal transit time was increased with a 15 min glutamate exposure. After 60 more minutes, the average time used by the ribosomes to complete a polypeptide chain had almost returned to its initial level. These results strongly suggest that glutamate exerts an exquisite time-dependent translational control in glial cells, a process that might be critical for glia-neuron interactions.
机译：谷氨酸是脊椎动物脑中的主要兴奋性递质，参与神经元发育和突触可塑性。谷氨酸能刺激导致神经元和神经胶质细胞中基因表达模式的差异。已经建立了几个基因的谷氨酸依赖性转录控制。但是，关于分子事件在接触该神经递质后会改变翻译机制的知识知之甚少。在小脑培养的Bergmann细胞的神经胶质模型中，谷氨酸诱导对[（35）S]-蛋氨酸掺入蛋白质的双相作用，这表明蛋白质生物合成的延长阶段是调控的目标。实际上，已经报道了在谷氨酸暴露15分钟后，伸长因子2磷酸化的瞬时增加，该作用是通过激活伸长因子2激酶介导的。在这项贡献中，我们试图表征谷氨酸暴露下的真核生物延伸因子1A（eEF1A）的磷酸化状态和核糖体转运时间。谷氨酸盐处理60分钟后发现eEF1A磷酸化呈剂量依赖性增加。此现象是Ca（2 +）/ CaM依赖性的，被Src和磷脂酰肌醇3激酶抑制剂和雷帕霉素阻断。同时，谷氨酸暴露15分钟会增加核糖体的转运时间。再过60分钟后，核糖体完成一条多肽链所用的平均时间几乎恢复到其初始水平。这些结果强烈表明，谷氨酸在神经胶质细胞中发挥着精细的时间依赖性翻译控制，这一过程可能对神经胶质-神经元的相互作用至关重要。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号