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首页> 外文期刊>Neurochemistry International: The International Journal for the Rapid Publication of Critical Reviews, Preliminary and Original Research Communications in Neurochemistry >Distribution of serotonin, its metabolites and 5-HT transporters in the neostriatum of Lurcher and weaver mutant mice.
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Distribution of serotonin, its metabolites and 5-HT transporters in the neostriatum of Lurcher and weaver mutant mice.

机译：血清素，其代谢物和5-HT转运蛋白在Lurcher和weaver突变小鼠的新纹状体中的分布。

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摘要

Serotonin (5-HT) uptake sites, or transporters, were measured in the neostriatum (caudate putamen) of wild type (+/+) mice and heterozygous (wv/+) and homozygous (wv/wv) weaver, as well as in heterozygous Lurcher (Lc/+) mutants. These topological surveys were carried out by quantitative ligand binding autoradiography using the uptake site antagonist [3H]-citalopram as a probe of innervation densities in four quadrants of the rostral neostriatum and in two halves of the caudal neostriatum. In addition, tissue concentrations of 5-HT, 5-hydroxyindole-3-acetic acid and 5-hydroxytryptophol were measured by high-performance liquid chromatography with electrochemical detection in these neostriatal divisions. In +/+ mice and in Lc/+ mutants there was a dorso-ventral gradient of increasing 5-HT levels, and they exhibited a similar heterogeneity of [3H]-citalopram labeling. In contrast, the gradients of 5-HT concentrations and [3H]-citalopram binding disappeared in the weaver mutants, suggesting a rearrangement of the 5-HT innervation. This reorganization of the 5-HT system in the neostriatum was more obvious in the wv/wv and is compatible with the hypothesis that the postnatal dopaminergic deficiencies that characterize weaver mutants lead to a sprouting of fibers and thus constitute a genetic model of dopaminergic denervation that leads to a 5-HT hyperinnervation.
机译：在野生型（+ / +）小鼠，杂合子（wv / +）和纯合子（wv / wv）的编织者的新纹状体（尾状壳）中测量了血清素（5-HT）的摄取位点或转运蛋白杂合Lurcher（Lc / +）突变体。这些拓扑学调查是通过定量配体结合放射自显影术进行的，使用摄取部位拮抗剂[3H]-西酞普兰作为神经纹状体的四个象限和尾状纹状体的两半的神经支配密度的探针。此外，在这些新纹状体区域中，通过高效液相色谱和电化学检测来测量5-HT，5-羟基吲哚-3-乙酸和5-羟基色酚的组织浓度。在+ / +小鼠和Lc / +突变体中，5-HT水平呈背腹梯度上升，并且它们表现出相似的[3H]-西酞普兰标记异质性。相比之下，5-HT浓度和[3H]-西酞普兰结合的梯度在weaver突变体中消失，表明5-HT神经支配的重排。 wv / wv中新纹状体中5-HT系统的这种重组在wv / wv中更为明显，并且与以下假设相吻合：假说是织造突变体的特征，即产后多巴胺能缺陷导致纤维萌芽，因此构成了多巴胺能神经支配的遗传模型，导致5-HT过度神经支配。

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