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首页> 外文期刊>Neurochemistry International: The International Journal for the Rapid Publication of Critical Reviews, Preliminary and Original Research Communications in Neurochemistry >Simultaneous monitoring of acetylcholine and catecholamine release in the in vivo rat adrenal medulla.
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Simultaneous monitoring of acetylcholine and catecholamine release in the in vivo rat adrenal medulla.

机译：同时监测体内大鼠肾上腺髓质中乙酰胆碱和儿茶酚胺的释放。

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摘要

To simultaneously monitor acetylcholine release from pre-ganglionic adrenal sympathetic nerve endings and catecholamine release from post-ganglionic adrenal chromaffin cells in the in vivo state, we applied microdialysis technique to anesthetized rats. Dialysis probe was implanted in the left adrenal medulla and perfused with Ringer's solution containing neostigmine (a cholinesterase inhibitor). After transection of splanchnic nerves, we electrically stimulated splanchnic nerves or locally administered acetylcholine through dialysis probes for 2 min and investigated dialysate acetylcholine, choline, norepinephrine and epinephrine responses. Acetylcholine was not detected in dialysate before nerve stimulation, but substantial acetylcholine was detected by nerve stimulation. In contrast, choline was detected in dialysate before stimulation, and dialysate choline concentration did not change with repetitive nerve stimulation. The estimated interstitial acetylcholine levels and dialysate catecholamine responses were almost identical between exogenous acetylcholine (10 microM) and nerve stimulation (2 Hz). Dialysate acetylcholine, norepinephrine and epinephrine responses were correlated with the frequencies of electrical nerve stimulation, and dialysate norepinephrine and epinephrine responses were quantitatively correlated with dialysate acetylcholine responses. Neither hexamethonium (a nicotinic receptor antagonist) nor atropine (a muscarinic receptor antagonist) affected the dialysate acetylcholine response to nerve stimulation. Microdialysis technique made it possible to simultaneously assess activities of pre-ganglionic adrenal sympathetic nerves and post-ganglionic adrenal chromaffin cells in the in vivo state and provided quantitative information about input-output relationship in the adrenal medulla.
机译：为了在体内状态下同时监测神经节前肾上腺交感神经末梢释放的乙酰胆碱和神经节后肾上腺嗜铬细胞释放儿茶酚胺，我们将微透析技术应用于麻醉的大鼠。将透析探针植入左肾上腺髓质中，并用含有新斯的明（胆碱酯酶抑制剂）的林格氏液灌注。横切内脏神经后，我们通过透析探针电刺激内脏神经或局部给予乙酰胆碱2分钟，并研究透析液中乙酰胆碱，胆碱，去甲肾上腺素和肾上腺素的反应。神经刺激前未在透析液中检出乙酰胆碱，但神经刺激后检出大量乙酰胆碱。相反，刺激之前在透析液中检测到胆碱，并且透析液胆碱浓度不会随着重复的神经刺激而改变。在外源性乙酰胆碱（10 microM）和神经刺激（2 Hz）之间，估计的间质性乙酰胆碱水平和透析液儿茶酚胺反应几乎相同。透析液的乙酰胆碱，去甲肾上腺素和肾上腺素的反应与电神经刺激的频率相关，而透析液的去甲肾上腺素和肾上腺素的反应与透析液中的乙酰胆碱的反应在数量上相关。六甲铵（烟碱样受体拮抗剂）和阿托品（毒蕈碱样受体拮抗剂）都不会影响对神经刺激的透析液乙酰胆碱的反应。微透析技术使在体内状态下同时评估神经节前肾上腺交感神经和神经节后肾上腺嗜铬细胞的活动成为可能，并提供了有关肾上腺髓质中输入输出关系的定量信息。

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