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首页> 外文期刊>Neurochemistry International: The International Journal for the Rapid Publication of Critical Reviews, Preliminary and Original Research Communications in Neurochemistry >Hyperbaric and normobaric reoxygenation of hypoxic rat brain slices--impact on purine nucleotides and cell viability.
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Hyperbaric and normobaric reoxygenation of hypoxic rat brain slices--impact on purine nucleotides and cell viability.

机译：低氧大鼠脑片的高压和常压复氧-影响嘌呤核苷酸和细胞活力。

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Hyperbaric oxygen treatment has been suggested as able to reduce hypoxia induced neuronal damage. The aim of the study was to compare the impact of different reoxygenation strategies on early metabolical (purine nucleotide content determined by HPLC) and morphological changes (index of cell injury after celestine blue/acid fuchsin staining) of hypoxically damaged rat neocortical brain slices. For this purpose slices (300 microm and 900 microm) were subjected to either 5 or 30 min of hypoxia by gassing the incubation medium with nitrogen. During the following reoxygenation period treatment groups were administered either 100% oxygen (O) or room air (A) at normobaric (1 atm absolute, NB-O; NB-A) or hyperbaric (2.5 atm absolute, HB-O; HB-A) conditions. After 5 min of hypoxia, both HB-O and NB-O led to a complete nucleotide status restoration (ATP/ADP; GTP/GDP) in 300 microm slices. However, reoxygenation after 30 min of hypoxia was less effective, irrespective of the oxygen pressure. Furthermore, administering hyperbaric room air resulted in no significant posthypoxic nucleotide recovery. In 900 microm slices, both control incubation as well as 30 min of hypoxia resulted in significantly lower trinucleotide and higher dinucleotide levels compared to 300 microm slices. While there was no significant difference between HB-O and NB-O on the nucleotide status, morphological evaluation revealed a better recovery of the index of cell injury (profoundly injured/intact cell-ratio) in the HB-O group. Conclusively, the posthypoxic recovery of metabolical characteristics was dependent on the duration of hypoxia and slice thickness, but not on the reoxygenation pressure. A clear restorative effect on purine nucleotides was found only in early-administered HB-O as well as NB-O in contrast to room air treated slices. However, these pressure independent metabolic changes were morphologically accompanied by a significantly improved index of cell injury, indicating a possible neuroprotective role of HB-O in early posthypoxic reoxygenation.
机译：高压氧治疗已被认为能够减少缺氧引起的神经元损伤。该研究的目的是比较不同的复氧策略对缺氧损伤的大鼠新皮层脑切片的早期代谢（通过HPLC测定嘌呤核苷酸含量）和形态变化（在天青蓝/酸性品红染色后细胞损伤指数）的影响。为此目的，通过向培养液中通入氮气，使切片（300微米和900微米）缺氧5或30分钟。在接下来的复氧期间，在常压（1 atm绝对值，NB-O; NB-A）或高压（2.5 atm绝对值，HB-O; HB- A）条件。缺氧5分钟后，HB-O和NB-O均可在300微米切片中导致核苷酸状态完全恢复（ATP / ADP; GTP / GDP）。但是，无论氧气压力如何，缺氧30分钟后的复氧效果都较差。此外，施用高压室内空气不会导致明显的缺氧后核苷酸恢复。在900微米切片中，与300微米切片相比，对照培养以及30分钟的缺氧均导致三核苷酸水平明显降低，而二核苷酸水平则升高。虽然HB-O和NB-O在核苷酸状态上没有显着差异，但形态学评估显示HB-O组的细胞损伤指数（深刻损伤/完整细胞比率）恢复得更好。总之，低氧后代谢特征的恢复取决于缺氧的持续时间和切片厚度，而不取决于复氧压力。与室内空气处理的切片相比，仅在早期给药的HB-O和NB-O中才发现对嘌呤核苷酸具有明显的修复作用。然而，这些与压力无关的代谢变化在形态上伴随着细胞损伤指数的显着改善，表明HB-O在早期低氧后复氧中可能具有神经保护作用。

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