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首页> 外文期刊>Neurochemistry International: The International Journal for the Rapid Publication of Critical Reviews, Preliminary and Original Research Communications in Neurochemistry >Pharmacological properties of glycine uptake in the developing rat retina.
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Pharmacological properties of glycine uptake in the developing rat retina.

机译：发育中的大鼠视网膜中甘氨酸摄取的药理特性。

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摘要

A pharmacological characterization of glycine transport was performed in the rat retina at different postnatal ages. The uptake of 3H-glycine increased during the first 2 weeks of postnatal age, reaching maximum values at 12 days; then it decreased sharply to the adult values. We found a Na+ -dependent and high-affinity transport system with a Km of 100 microM. The Na+ Hill coefficient for glycine uptake was 1.76 +/- 0.07. Although glycine uptake was insensitive to staurosporine and phorbol ester, it was reduced 40-50% by sarcosine and ALX5407. Besides, amoxapine inhibited glycine uptake by 40 and 70% in adult and immature retina, respectively. These results suggest that the Glyt1 transporter was concentrated in the nerve terminals. In addition to the presence of Glyt1 in the retina, our results provided evidence of the occurrence of Glyt2 and/or another isoform of glycine transporter, which might have had a role in the retina development.
机译：在不同出生年龄的大鼠视网膜中进行了甘氨酸转运的药理学表征。在出生后的前两周，3H-甘氨酸的摄取增加，在12天达到最大值。然后它急剧下降到成人值。我们发现了Na +依赖性的高亲和力转运系统，其Km为100 microM。甘氨酸摄取的Na + Hill系数为1.76 +/- 0.07。尽管甘氨酸的吸收对星形孢菌素和佛波酯不敏感，但肌氨酸和ALX5407使甘氨酸的吸收减少了40-50％。此外，阿莫沙平在成年和未成熟视网膜中分别抑制了40％和70％的甘氨酸吸收。这些结果表明Glyt1转运蛋白集中在神经末梢。除了在视网膜中存在Glyt1之外，我们的结果还提供了Glyt2和/或甘氨酸转运蛋白的另一种同工型的发生的证据，这可能与视网膜的发育有关。

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