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首页> 外文期刊>Neurochemistry International: The International Journal for the Rapid Publication of Critical Reviews, Preliminary and Original Research Communications in Neurochemistry >High glucose and diabetes increase the release of (3H)-D-aspartate in retinal cell cultures and in rat retinas.
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High glucose and diabetes increase the release of (3H)-D-aspartate in retinal cell cultures and in rat retinas.

机译：高血糖和糖尿病会增加（3H）-D-天门冬氨酸在视网膜细胞培养物中和大鼠视网膜中的释放。

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摘要

Several evidences suggest that glutamate may be involved in retinal neurodegeneration in diabetic retinopathy (DR). For that reason, we investigated whether high glucose or diabetes affect the accumulation and the release of [(3)H]-D-aspartate, which was used as a marker of the glutamate transmitter pool. The accumulation of [(3)H]-D-aspartate did not change in cultured retinal neural cells treated with high glucose (30 mM) for 7 days. However, the release of [(3)H]-D-aspartate, evoked by 50 mM KCl, significantly increased in retinal cells exposed to high glucose. Mannitol, which was used as an osmotic control, did not cause any significant changes in both accumulation and release of [(3)H]-D-aspartate. In the retinas, 1 week after the onset of diabetes, both the accumulation and release of [(3)H]-D-aspartate were unchanged comparing to the retinas of age-matched controls. However, after 4 weeks of diabetes, the accumulation of [(3)H]-D-aspartate in diabetic retinas decreased and the release of [(3)H]-D-aspartate increased, compared to age-matched control retinas. These results suggest that high glucose and diabetes increase the evoked release of D-aspartate in the retina, which may be correlated with the hypothesis of glutamate-induced retinal neurodegeneration in DR.
机译：一些证据表明，谷氨酸可能与糖尿病性视网膜病（DR）的视网膜神经变性有关。因此，我们调查了高血糖或糖尿病是否会影响[（3）H] -D-天门冬氨酸的积累和释放，这被用作谷氨酸传输池的标志物。 [（3）H] -D-天冬氨酸的积累在高葡萄糖（30 mM）处理7天的视网膜神经细胞中未改变。但是，[（3）H] -D-天门冬氨酸的释放，由50 mM KCl引起，在暴露于高葡萄糖的视网膜细胞中显着增加。甘露醇，用作渗透控制，没有引起[（3）H] -D-天冬氨酸的积累和释放的任何重大变化。在糖尿病发病后1周的视网膜中，[（3）H] -D-天门冬氨酸的积累和释放与年龄相匹配的对照组的视网膜相比均未改变。然而，与年龄匹配的对照视网膜相比，糖尿病4周后，[（3）H] -D-天冬氨酸在糖尿病视网膜中的积累减少，[[3）H] -D-天冬氨酸的释放增加。这些结果表明，高血糖和糖尿病会增加视网膜中D-天冬氨酸的诱发释放，这可能与DR中谷氨酸诱导的视网膜神经变性的假说有关。

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