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首页> 外文期刊>Neurochemistry International: The International Journal for the Rapid Publication of Critical Reviews, Preliminary and Original Research Communications in Neurochemistry >Transient global ischemia in rat brain promotes different NMDA receptor regulation depending on the brain structure studied.
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Transient global ischemia in rat brain promotes different NMDA receptor regulation depending on the brain structure studied.

机译：根据研究的大脑结构，大鼠大脑中的短暂性全局缺血促进不同的NMDA受体调节。

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The mRNA expression of the major subunits of N-methyl-d-aspartate receptors (NR1, NR2A and NR2B) following ischemia-reperfusion was studied in structures with different vulnerabilities to ischemic insult in the rat brain. The study was performed using quantitative real-time PCR on samples from 3-month-old male Sprague-Dawley rats after global transient forebrain ischemia followed by 48h of reperfusion. Expression of NMDA receptor subunits mRNAs decreased significantly in all structures studied in the injured animals as compared to the sham-operated ones. The hippocampal subfields (CA1, CA3 and dentate gyrus) as well as the caudate-putamen, both reported to be highly ischemic-vulnerable structures, showed outstandingly lower mRNA levels of NMDA receptor subunits than the cerebral cortex, which is considered a more ischemic-resistant structure. The ratios of the mRNA levels of the different subunits were analyzed as a measure of the NMDA receptor expression pattern for each structure studied. Hippocampalareas showed changes in NMDA receptor expression after the insult, with significant decreases in the NR2A with respect to the NR1 and NR2B subunits. Thus, the NR1:NR2A:NR2B (1:1:2) ratios observed in the sham-operated animals became (2:1:4) in insulted animals. This modified expression pattern was similar in CA1, CA3 and the dentate gyrus, in spite of the different vulnerabilities reported for these hippocampal areas. In contrast, no significant differences in the expression pattern were observed in the caudate-putamen or cerebral cortex on comparing the sham-operated animals with the ischemia-reperfused rats. Our results support the notion that the regulation of NMDA receptor gene expression is dependent on the brain structure rather than on the higher or lower vulnerability of the area studied.
机译：研究了大鼠脑缺血再灌注不同易损性结构中N-甲基-d-天冬氨酸受体主要亚基（NR1，NR2A和NR2B）的mRNA表达。这项研究是使用实时定量PCR对3个月大的雄性Sprague-Dawley大鼠的样本进行的，该样本在整体短暂性前脑缺血后再灌注48h。与假手术组相比，在受损动物中研究的所有结构中，NMDA受体亚基的mRNA表达均显着下降。据报道，海马亚区（CA1，CA3和齿状回）以及尾状丘脑都具有高度缺血性的结构，其NMDA受体亚基的mRNA水平显着低于大脑皮层，后者被认为是缺血性更强的。抗性结构。分析了不同亚基的mRNA水平的比率，以作为所研究每种结构的NMDA受体表达模式的量度。海马体在受到伤害后显示NMDA受体表达发生变化，相对于NR1和NR2B亚基而言，NR2A显着下降。因此，在假手术动物中观察到的NR1∶NR2A∶NR2B（1∶1∶2）的比例在受侮辱的动物中变为（2∶1∶4）。尽管在这些海马区报告了不同的脆弱性，但这种修饰的表达模式在CA1，CA3和齿状回中相似。相反，将假手术动物与缺血再灌注大鼠进行比较时，在尾状丘脑或大脑皮层中未观察到表达模式的显着差异。我们的结果支持以下观点：NMDA受体基因表达的调节取决于大脑结构，而不是所研究区域的较高或较低的脆弱性。

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