...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Neurochemistry International: The International Journal for the Rapid Publication of Critical Reviews, Preliminary and Original Research Communications in Neurochemistry >DHA enhances the noradrenaline release by SH-SY5Y cells.
【24h】

DHA enhances the noradrenaline release by SH-SY5Y cells.

机译：DHA增强了SH-SY5Y细胞释放去甲肾上腺素的能力。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Polyunsaturated fatty acids (PUFAs), particularly docosahexaenoic acid (DHA) and arachidonic acid (AA), are the main components of the phospholipids, in cerebral membranes. A dietary-induced cerebral DHA deficit results in altered behaviour and neurotransmission in rodents. To determine whether PUFA were acting on the neurotransmitter release machinery, we measured the release of [(3)H]-noradrenaline (NA) from SH-SY5Y neuroblastoma cells with modified PUFA membrane contents and from cells incubated with medium containing high DHA or AA. The membranes of cells incubated with 70 microM DHA for 3 days had 7.6-times more DHA in their ethanolamine glycerophospholipids, while the membranes of cells incubated with AA had 40% less. Incorporation of DHA enhanced basal [(3)H]-NA release (25%, p<0.05), but not KCl-evoked [(3)H]-NA release. Brief incubation with DHA during vesicle mobilization also strongly increased [3H]-NA release. AA had no effect. The genes encoding for the calcium sensor synaptotagmin 1, and for the two SNARE complex proteins syntaxin 1A and synaptobrevin 1 were not affected by PUFA incorporation, as indicated by assays for specific mRNAs and proteins. Thus both a high membrane DHA content and free DHA in the medium enhance the release of [(3)H]-NA from SH-SY5Y cells. This suggests that brain membrane DHA influences exocytosis, which then regulates neurotransmission.
机译：多不饱和脂肪酸（PUFA），尤其是二十二碳六烯酸（DHA）和花生四烯酸（AA）是脑膜中磷脂的主要成分。饮食引起的大脑DHA缺乏会导致啮齿动物行为和神经传递发生改变。为了确定PUFA是否作用于神经递质释放机制，我们测量了具有修饰的PUFA膜含量的SH-SY5Y神经母细胞瘤细胞和含有高DHA或AA的培养基培养的细胞的[（3）H]-去甲肾上腺素（NA）释放。用70 microM DHA培养3天的细胞膜的乙醇胺甘油磷脂中的DHA高7.6倍，而用AA培养的细胞膜的DHA少40％。掺入DHA增强了基础[（3）H] -NA的释放（25％，p <0.05），但没有KCl诱发的[（3）H] -NA的释放。在小泡动员期间与DHA短暂孵育也大大增加了[3H] -NA的释放。 AA没有影响。如针对特定mRNA和蛋白质的检测所表明的，编码钙传感器突触小分子1和两种SNARE复杂蛋白的语法Syntaxin 1A和突触短纤维蛋白1的基因不受PUFA掺入的影响。因此，培养基中的高膜DHA含​​量和游离DHA均可增强[（3）H] -NA从SH-SY5Y细胞的释放。这表明脑膜DHA影响胞吐作用，进而调节神经传递。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号