...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Neurochemistry International: The International Journal for the Rapid Publication of Critical Reviews, Preliminary and Original Research Communications in Neurochemistry >Differential regulation of C-terminal splice variants of the glutamate transporter GLT-1 by tumor necrosis factor-alpha in primary cultures of astrocytes
【24h】

Differential regulation of C-terminal splice variants of the glutamate transporter GLT-1 by tumor necrosis factor-alpha in primary cultures of astrocytes

机译：星形胶质细胞原代培养物中肿瘤坏死因子-α对谷氨酸转运蛋白GLT-1的C端剪接变体的差异调节。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The high-affinity glutamate transporter GLT-1 plays a key role in the control of the glutamate homeostasis in the central nervous system and protects neurons against excitotoxicity. Splice variants of the original transcript have been identified and their involvement in neurodegenerative disorders has been proposed. However, the functions and the regulations of these isoforms remain unclear. In this study, we focused our interest on the expression of two C-terminal splice variants of GLT-1 (GLT-1 a and b) in primary astrocyte cultures exposed to distinct chemical environments. While GLT-la and GLT-lb mRNAs were both increased in response to treatment with N6,2'-O-dibutyryladenosine 3',5'-cyclic monophosphate (dBcAMP), the culture supplement G5 or tumor necrosis factor-alpha (TNF-alpha), the regulation of GLT-1b appeared quicker and was more pronounced. Besides, using validated antibodies, we evidenced a differential regulation of the two proteins in cells exposed to TNF-alpha. Thus, while dBcAMP and the G5 supplement stimulated the expression of both isoforms at 3 and 7 days, a transient upregulation of GLT-1a was induced by TNF-alpha, which contrasts with the sustained induction of the GLT-1b isoform. These results shed light on the complex influence of the pro-inflammatory cytokine TNF-ct on GLT-la mRNA and protein expression and on the necessity to distinctly consider the GLT-1 isoforms with appropriate tools in studies addressing the regulation of glutamate transporters.
机译：高亲和力谷氨酸转运蛋白GLT-1在控制中枢神经系统中的谷氨酸稳态中起关键作用，并保护神经元免受兴奋性毒性作用。已经鉴定出原始转录本的剪接变体，并提出了它们参与神经退行性疾病的提议。但是，这些同工型的功能和规定尚不清楚。在这项研究中，我们的兴趣集中在暴露于不同化学环境的原代星形胶质细胞培养物中GLT-1的两个C端剪接变体（GLT-1 a和b）的表达。虽然GLT-1a和GLT-1b mRNA均因N6,2'-O-二丁酰腺苷3'，5'-环一磷酸（dBcAMP），培养补充G5或肿瘤坏死因子-α（TNF- α），GLT-1b的调节出现得更快，更明显。此外，使用经过验证的抗体，我们证明了暴露于TNF-α的细胞中两种蛋白质的差异调节。因此，尽管dBcAMP和G5补充物在第3天和第7天刺激了两种同工型的表达，但TNF-α诱导了GLT-1a的瞬时上调，这与持续诱导GLT-1b同型相反。这些结果阐明了促炎性细胞因子TNF-ct对GLT-1αmRNA和蛋白质表达的复杂影响，以及在研究谷氨酸转运蛋白调控的研究中需要用适当的工具清楚地考虑GLT-1同工型的必要性。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号