...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Neurochemistry International: The International Journal for the Rapid Publication of Critical Reviews, Preliminary and Original Research Communications in Neurochemistry >Regulation of ecto-5′-nucleotidase (CD73) in cultured cortical astrocytes by different inflammatory factors
【24h】

Regulation of ecto-5′-nucleotidase (CD73) in cultured cortical astrocytes by different inflammatory factors

机译：不同炎症因子对皮质星形胶质细胞中ecto-5′-核苷酸酶（CD73）的调节

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Ecto-5′-nucleotidase (e-5NT) is a cell-surface located, rate-limiting enzyme in the extracellular metabolism of ATP, catalyzing the final step of the conversion of AMP to adenosine. Since this enzyme shifts the balance from pro-inflammatory ATP to anti-inflammatory adenosine, it is considered to be an important regulator of inflammation. Although up-regulation of e-5NT was repeatedly reported in several in vivo models of brain injury, the regulation of its expression and function remains largely unknown. We have studied effects of several pro-inflammatory factors, namely, bacterial endotoxin lipopolysaccharide (LPS), tumor necrosis factor-α (TNF-α), interferon-γ (IFN-γ), glutamate (Glu) and hydrogen peroxide (H 2O 2) on e-5NT (i) activity, (ii) mRNA expression and (iii) membrane protein abundance in primary cultured cortical astrocytes. We are clearly able to demonstrate a stimulus-specific regulation of the e-5NT pathway. IFN-γ, LPS, Glu and H 2O 2 decrease, while TNF-α increases e-5NT activity. The analysis of e-5NT gene expression and e-5NT membrane protein levels revealed that tested factors regulate e-5NT at different levels and by employing different mechanisms. In summary, we provide evidence that e-5NT activity is tightly regulated in a stimulus-specific manner.
机译：Ecto-5'-核苷酸酶（e-5NT）是ATP胞外代谢中位于细胞表面的限速酶，催化AMP转化为腺苷的最后一步。由于该酶将平衡从促炎性ATP转变为消炎性腺苷，因此被认为是炎症的重要调节剂。尽管在几种脑损伤的体内模型中反复报道了e-5NT的上调，但其表达和功能的调节仍然未知。我们已经研究了几种促炎因子的作用，即细菌内毒素脂多糖（LPS），肿瘤坏死因子-α（TNF-α），干扰素-γ（IFN-γ），谷氨酸（Glu）和过氧化氢（H 2O） 2）关于e-5NT（i）活性，（ii）原代培养的皮质星形胶质细胞中的mRNA表达和（iii）膜蛋白丰度。我们显然能够证明e-5NT途径的刺激特异性调节。 IFN-γ，LPS，Glu和H 2O 2降低，而TNF-α增加e-5NT活性。对e-5NT基因表达和e-5NT膜蛋白水平的分析表明，受试因子以不同水平和采用不同机制调节e-5NT。总之，我们提供的证据表明，e-5NT的活性受到刺激特异性方式的严格调控。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号