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首页> 外文期刊>Neurochemistry International: The International Journal for the Rapid Publication of Critical Reviews, Preliminary and Original Research Communications in Neurochemistry >Regional distribution and relative amounts of glutamate decarboxylase isoforms in rat and mouse brain.
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Regional distribution and relative amounts of glutamate decarboxylase isoforms in rat and mouse brain.

机译：大鼠和小鼠脑中谷氨酸脱羧酶同工型的区域分布和相对量。

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摘要

The levels of the two isoforms of glutamate decarboxylase (GAD) were measured in 12 regions of adult rat brain and three regions of mouse brain by sodium dodecylsulfate-polyacrylamide gel electrophoresis and immunoblotting with an antiserum that recognizes the identical C-terminal sequence in both isoforms from both species. In rat brain the amount of smaller isoform, GAD65, was greater than that of the larger isoform, GAD67, in all twelve regions. GAD65 ranged from 77-89% of total GAD in frontal cortex, hippocampus, hypothalamus, midbrain, olfactory bulb, periaqueductal gray matter, substantia nigra, striatum, thalamus and the ventral tegmental area. The proportion of GAD65 was lower in amygdala and cerebellum but still greater than half of the total. There was a strong correlation between total GAD protein and GAD activity. In the three mouse brain regions analysed (cerebellum, cerebral cortex and hippocampus) the proportion of GAD65 (35,47, and 51% of total GAD) was significantly lower than in the corresponding rat-brain regions. The amount of GAD67 was greater than the amount of GAD65 in mouse cerebellum and was approximately equal to the amount of GAD65 in mouse cerebral cortex and hippocampus.
机译：通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳和抗血清免疫印迹法检测成年大鼠大脑的12个区域和小鼠大脑的三个区域中的两种谷氨酸脱羧酶（GAD）的水平，并用一种​​可识别两种亚型相同C端序列的抗血清进行免疫印迹来自两个物种。在大鼠大脑中，在所有十二个区域中，较小的同工型GAD65的数量均大于较大的同工型GAD67的数量。在额叶皮质，海马，下丘脑，中脑，嗅球，导水管周围灰质，黑质，黑质，纹状体，丘脑和腹侧被盖区，GAD65占总GAD的77-89％。杏仁核和小脑中GAD65的比例较低，但仍占总比例的一半以上。总GAD蛋白与GAD活性之间存在很强的相关性。在所分析的三个小鼠大脑区域（小脑，大脑皮层和海马）中，GAD65的比例（分别占总GAD的35,47和51％）显着低于相应的大鼠脑区域。 GAD67的量大于小鼠小脑中的GAD65量，大约等于小鼠大脑皮层和海马中的GAD65量。

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